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Ensiling phases. E. coli Toxins!. Clostridia ! anaerob! ..but pH sensitive!. L. monocytogenes gram-positive facultative anaerobic IN SPOILED SILAGES!. Yeast Aerobic insta-bility!. Moulds Myco-toxins!. additives. Silage additives Stimulants inhibitors substrate suppliers anaerobic
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Ensilingphases E. coli Toxins! Clostridia! anaerob! ..but pH sensitive! L. monocytogenes gram-positive facultative anaerobic IN SPOILED SILAGES! Yeast Aerobic insta-bility! Moulds Myco-toxins!
additives Silage additives Stimulantsinhibitors substratesuppliersanaerobic Inoculantenzymessugarsaerobicacidsothers LacticacidcellulasemolassespropionicacidformicSulphurdioxyde Bacteriaamylasesucrosessulfatesmineralformaldehyde hemicellulasesglucosescaproicacidlacticSodiumbisph proteasessorbicacidacetic pectinasesammoniabenzoic aceticacidacrylic propionatescytric sorbic
Biomin®BioStabil blend of different homofermentative and heterofermentative bacteria for optimal anaerobic and aerobic stability of silages lactic acid acetic acid anaerobic stability aerobic stability
Bacteria Homofermentative heterofermentative Lactobacillusacidophilus L. Brevis L.Casei L. Buchnerii L Coryniformis L. cellobiosios L Planterum L fermentum L salivarius L viridescens Pediococcusacidilatictic P damnosus EnterococcusFaecium e. Faecium lactobacillus
1. Selectionofthebestsilagestrainsfortheproduct „GRAS“ status Antibiotic resistance Risk analysis Virulence activity strainidentity • Isolation and characterization • of bacteria out of silages • Evaluation and selection of most effective strains • Productionoflacticacid (high amount / very fast) • Productionofaceticacid • Ratio lactic/aceticacid (differentiation homo- and heterofermentative) • Goodgrowth in a broad pH range • Goodefficacy in a broadrangeofforagesandgrains • Safety • Risk assessment • Stability • Fermentation performance • Highest product quality
1. Evaluation and strain selection • Range of metabolic end products • Production of lactic acid (high amount / very fast) • Production of acetic acid • Ratio lactic/acetic acid • (differentiation homo- and heterofermentative) • Good growth in a broad pH range • Good efficacy in a broad range of forages and grains
Biomin®BioStabil Selected strains: • for different substrates • for different energy sources • more stable Multistrain Lactobacilus plantarum Enterococcus faecium Lactobacillus brevis Criteria for selection
Biomin®BioStabil Enterococcus faecium Lactobacillus plantarum Lactobacillus brevis
DM LOSSES moisture% fillingseepage gas Top-spoilFeedout Total Based on Forages: The Science of Grassland Agriculture, 4th ed. See Bickert et al (1997
Silage Average Dairy Farm: 500 cows + young stock. Corn silage : 8300 t ( 2900 t DM) @ R500/ton Hay : 700 t ( 570 t DM) @ R700/ton Total costs/value: (8300 ton*R500/t)+(700 ton*R700/t)=4,6 mln R
Dry matter losses Losses are; Best case: 17 % 4,6 mln R *17% = 782.000 R or Worst case: 30%4,6mln R* 30% = 1,38 mln R
BioStabil effect on Energy and DM Values that can be expected provided good management practises: 1 Mcal = 4,184 MJ Energy (NEL, Mcal)/kg milk: 0,72511
Energy losses Corn silage 2900 t DM= 18.4 mMj recovery Max Losses 30% = 5520000 MJ Min Losses 5 % = 920000 MJ OR 540 T extra dry corn!
economics Cornsilage 2900 t DM: Energyrecovery: 0,18 Mj/kg dm 1500000 kg*0,18 Mj= 270.000 Mj. Corn= 8,5 Mj = 61 t Corn=150.000 R Withouttakingtheproteinquality in consideration
Questions? Onno.breitsma@biomin.net