1 / 25

Loschmidt Laboratories Enantis , Ltd .

PROTEIN ENGINEERING. Loschmidt Laboratories Enantis , Ltd. LOSCHMIDT LABORATORIES & ENANTIS. 17 years experience in protein science multi - disciplinary team. LOSCHMIDT LABORATORIES & ENANTIS. 17 years experience in protein science multi - disciplinary team

jada
Download Presentation

Loschmidt Laboratories Enantis , Ltd .

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. PROTEIN ENGINEERING LoschmidtLaboratories Enantis, Ltd.

  2. LOSCHMIDT LABORATORIES & ENANTIS • 17 years experience in protein science • multi-disciplinary team

  3. LOSCHMIDT LABORATORIES & ENANTIS • 17 years experience in protein science • multi-disciplinary team • laboratories and facilities(1.5 mil EUR)University Campusand Biotechnology Incubator, Brno

  4. LOSCHMIDT LABORATORIES & ENANTIS • 17 years experience in protein science • multi-disciplinary team • laboratories and facilities(1.5 mil EUR) University Campusand Biotechnology Incubator, Brno • molecular biology andmicrobiology

  5. LOSCHMIDT LABORATORIES & ENANTIS • 17 years experience in protein science • multi-disciplinary team • laboratories and facilities(1.5 mil EUR) University Campusand Biotechnology Incubator, Brno • molecular biology andmicrobiology • bioinformaticsandmolecular modelling

  6. LOSCHMIDT LABORATORIES & ENANTIS • 17 years experience in protein science • multi-disciplinary team • laboratories and facilities(1.5 mil EUR) University Campusand Biotechnology Incubator, Brno • molecular biology andmicrobiology • bioinformaticsandmolecular modelling • biochemistryandbiophysics

  7. LOSCHMIDT LABORATORIES & ENANTIS BIOSENSING BIOCATALYSIS DECONTAMINATION

  8. DEVELOPMENT OF BIOCATALYTIC PROCESS

  9. DEVELOPMENT OF BIOCATALYTIC PROCESS • GENETIC DATABASES • EXTREMOPHILES

  10. DEVELOPMENT OF BIOCATALYTIC PROCESS • DIRECTED EVOLUTION • resistance to organicsolvents

  11. DEVELOPMENT OF BIOCATALYTIC PROCESS • DIRECTED EVOLUTION • RATIONAL DESIGN • thermostabilityenhancement

  12. DEVELOPMENT OF BIOCATALYTIC PROCESS • DIRECTED EVOLUTION • RATIONAL DESIGN • thermostabilityenhancement

  13. DEVELOPMENT OF BIOCATALYTIC PROCESS • DIRECTED EVOLUTION • RATIONAL DESIGN • thermostabilityenhancement • 20°C

  14. DEVELOPMENT OF BIOCATALYTIC PROCESS • OPTIMIZED EXPRESSION • FERMENTATION • DOWN STREAM

  15. DEVELOPMENT OF BIOCATALYTIC PROCESS • MEDIUM ENGINEERING • IMMOBILISATION

  16. HOTSPOT WIZARD RCSB PDB Hot Spot Wizard user

  17. HOTSPOT WIZARD CSA PDBSWS UniProt RCSB PDB Hot Spot Wizard user

  18. HOTSPOT WIZARD CSA PDBSWS UniProt RCSB PDB CASTp Hot Spot Wizard user CAVER

  19. HOTSPOT WIZARD CSA PDBSWS UniProt RCSB PDB CASTp Hot Spot Wizard user CAVER Rate4Site Cd-hit MUSCLE tunnels BLAST NRDB

  20. low high mutability HOTSPOT WIZARD

  21. HOTSPOT WIZARD haloalkanedehalogenase PDB-ID 1BN6 1,3-1,4-b-glucanase PDB-ID 1GBG b-lactamase PDB-ID 1BLS phosphotriesterase PDB-ID 1I0D

  22. HOTSPOT WIZARD positive and neutral hot spots region Proportion of mutations Estimated mutability

  23. HOTSPOT WIZARD positive and neutral slightly negative hot spots region Proportion of mutations Estimated mutability

  24. HOTSPOT WIZARD positive and neutral slightly negative deleterious hot spots region Proportion of mutations Estimated mutability

  25. HOTSPOT WIZARD Pavelkaet al. Nucl. Acids Res. 37: W376 2009

More Related