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Supplementary Figure 1

Supplementary Figure 1. B. Control X0. 100 90 80 70 60 50 40 30 20 10. a - HBx ( mAb ). Control X9. a - HBx (rabbit serum). C. A. B. C. E. F. 0. X0. X0. X0. X0. X0. X0. X0. X0. X9. X9. X9. X9. X9. X9. X9. X9. Control. Control. Control. Control.

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Supplementary Figure 1

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  1. Supplementary Figure 1 B Control X0 100 90 80 70 60 50 40 30 20 10 a-HBx (mAb) Control X9 a-HBx (rabbit serum) C A B C E F 0 X0 X0 X0 X0 X0 X0 X0 X0 X9 X9 X9 X9 X9 X9 X9 X9 Control Control Control Control Control Control Control Control Cells (%) A 1 58 85 120 154 aa A B C D E F X0 X9 (DD) 0 1 2 3 4 6 8 10 Post Double Thymidine Block (h)

  2. Supplementary Figure 2 A + 1 AP-2 CCAAT NFkb + 225 -1534 E2F1 E2F1 CDC6 promoter [-1534, +225] - 100 - 200 -130 Wild type E2F1 binding site TTTGGCGC Luciferase E2F1 E2F1 CDC6 WT -130 Mutant E2F1 binding site TTTAACGC E2F1 E2F1 Luciferase CDC6-SM1 B # # Control X0 Normalized luciferase units pGL3 CDC6 WT CDC6-SM1 C Cyclin A Binding (68-108) DP-1 Binding (206-283) DNA Binding (120-191) Rb binding Transactivation Domain (323-437) Wild-type E2F1-WT Transactivation Domainmutant E2F1 (1-374)

  3. Supplementary Figure 3 + 1 CCAAT CCAAT CCAAT Sp1 Sp1 Sp1 Sp1 Sp1 -728 + 127 E2F1 promoter [-728, +127] E2F1 E2F1 - 122 - 204 - 242 - 242 Luciferase E2F1 E2F1 pGL3-E2F1 WT - 242 Luciferase E2F1 E2F1 pGL3-E2F1 Mut

  4. Supplementary Figure 4 A Human b-globin origin Relative abundance of CDC6 on human b-globin origin of replication (arbitrary unit) ChIP Control X0 X9 Control X0 X9 a-CDC6 Control Serum Stimulation (h) Input 3h 7h Serum Stimulation B g-globulin non-origin locus Control X0 X9 Control X0 X9 ChIP a-CDC6 Relative abundance of CDC6 on human g-globulin locus (arbitrary unit) Control Input 3h 7h Serum Stimulation Serum Stimulation (h)

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