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Study of Electroosmosis in High Temperature Electrophoretic Measurements in Aqueous Solutions

Study of Electroosmosis in High Temperature Electrophoretic Measurements in Aqueous Solutions. Víctor Rodríguez-Santiago EGEE 520. Background. Electrophoresis What? Movement of charged particles in aqueous solution under the influence of an external electric field. Uses?

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Study of Electroosmosis in High Temperature Electrophoretic Measurements in Aqueous Solutions

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  1. Study of Electroosmosis in High Temperature Electrophoretic Measurements in Aqueous Solutions Víctor Rodríguez-Santiago EGEE 520

  2. Background • Electrophoresis What? Movement of charged particles in aqueous solution under the influence of an external electric field. Uses? Protein separation, DNA migration studies, contaminant detection and separation. • Electroosmosis What? Movement of fluid under the influence of an external electric field. (Undesired in electrophoretic measurements)

  3. Background • Methods to prevent electroosmosis Polymeric coatings Buffer solutions Radial electric fields (Do not work for high temperatures) • Modeling to quantify electroosmosis • What causes electroosmosis? Electrical Double Layer

  4. Governing Equations • Using COMSOL™: Navier-Stokes mode Electrostatic mode Combined

  5. Solution Stagnant Layer Back flow Electroosmotic flow

  6. Validation • Experimental data was used to validate the model. With the modeling data, the experimental electrophoretic data can be corrected for electroosmosis. At pH 7: Experimental electrophoretic data: 1.76E-8 m2V-1s-1 Electroosmotic modeled data: 2.09E-9 m2V-1s-1 Corrected data: 1.55E-8 m2V-1s-1 The corrected value is well within the confidence value of the experimental data.

  7. Parametric Study

  8. Parametric Study

  9. Concluding Remarks • The COMSOL simulation gives reasonable values for the electroosmotic mobility. • When used to correct for the experimental data, the correction falls within the confidence values. • Either the electroosmotic effect is small or the simulation underestimates its value.

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