Hybrid Multi-Mode Microplate Reader

1. Hybrid Multi-Mode Microplate Reader

2. Contents • Synergy H4 – Hybrid Technology • Key Features and Benefits • Quadruple Grating Architecture • Variable Band-Pass Selection • High-Performance, Easy-to-Use Filter System • Automated Z-Focusing • Dual Reagent Dispenser Option • Temperature Control System to 65°C • Take3 Multi-Volume Multi-Sample Plate with 2 µL microspots • Specifications and Summary

3. Synergy™ H4: Hybrid Technology Filter-based optics: • Use of dichroic mirrors increases sensitivity about 5x compared to filter-only optics • Peak transmission for filters and mirrors is around 80%. Very efficient optical system • Because of high amount of energy delivered to samples, high sensitivity can be obtained with short read times • In summary, a fast and very sensitive optical system

4. Synergy™ H4: Hybrid Technology Monochromator-based optics: • Use of two gratings per monochromator provides smooth performance from 250 to 900 nm • Very large range of applications, including spectral scanning • Variable bandpass system adds flexibility • In summary, an extremely flexible optical system

5. Synergy™ H4: Hybrid Technology Patent-pending design: • Flexible monochromator system • High-performance filter system • Each with its strengths and weaknesses • Extremely versatile instrument

6. Contents • Synergy H4 – Hybrid Technology • Key Features and Benefits • Quadruple Grating Architecture • Variable Band-Pass Selection • High-Performance, Easy-to-Use Filter System • Automated Z-Focusing • Dual Reagent Dispenser Option • Temperature Control System to 65°C • Take3™ Multi-Volume Platewith 2 µL microspots • Specifications and Summary

7. Contents • Synergy H4 – Hybrid Technology • Key Features and Benefits • Quadruple Grating Architecture • Variable Band-Pass Selection • High-Performance, Easy-to-Use Filter System • Automated Z-Focusing • Dual Reagent Dispenser Option • Temperature Control System to 65°C • Take3™ Multi-Volume Plate with 2 µL microspots • Specifications and Summary

8. Contents • Synergy H4 – Hybrid Technology • Key Features and Benefits • Quadruple Grating Architecture • Variable Band-Pass Selection • High-Performance, Easy-to-Use Filter System • Automated Z-Focusing • Dual Reagent Dispenser Option • Temperature Control System to 65°C • Take3™ Multi-Volume Plate with 2 µL microspots • Specifications and Summary

9. Dichroic mirrors: • Easy side access • Easy load/unload software function Filter wheels: • Easy front access • No tool required • Easy load/unload software function High-performance, easy-to-use filter system

10. Contents • Synergy H4 – Hybrid Technology • Key Features and Benefits • Quadruple Grating Architecture • Variable Band-Pass Selection • High-Performance, Easy-to-Use Filter System • Automated Z-Focusing • Dual Reagent Dispenser Option • Temperature Control System to 65°C • Take3™ Multi-Volume Plate with 2 µL microspots • Specifications and Summary

11. Automated Z-Focusing Top fiber 300 µl 200 µl 100 µl If no Z-focusing available, performance issues in low volumes, or use of adapters required. 300 µl 200 µl With Z-focusing, reading height can be precisely adjusted (100 µm resolution) for best performance on all plate types, and with all volumes. 100 µl

12. Contents • Synergy H4 – Hybrid Technology • Key Features and Benefits • Quadruple Grating Architecture • Variable Band-Pass Selection • High-Performance, Easy-to-Use Filter System • Automated Z-Focusing • Dual Reagent Dispenser Option • Temperature Control System to 65°C • Take3™ Multi-Volume Plate with 2 µL microspots • Specifications and Summary

13. Baseline Response Trigger Reagent Injection Dual Reagent Dispenser Option • Two reagent dispensers • Volume range 5 µL - 1000 µL • Enables dispense/read processes • Ion channel assays, flash luminescent assays, time-sensitive kinetic assays

14. Contents • Synergy H4 – Hybrid Technology • Key Features and Benefits • Quadruple Grating Architecture • Variable Band-Pass Selection • High-Performance, Easy-to-Use Filter System • Automated Z-Focusing • Dual Reagent Dispenser Option • Temperature Control System to 65°C • Take3™ Multi-Volume Plate with 2 µL microspots • Specifications and Summary

15. 1 2 3 4 Advanced 4-ZoneTM Temperature Control System • 4 independent heaters with 4 monitoring sensors • Precision: +/- 0.5°C at 37°C • Range: RT to 65°C • Enables applications with strict temperature uniformity requirements (e.g. ORAC assays) • Enables high-temperature assays (e.g. Invader assays at 62°C)

16. Contents • Synergy H4 – Hybrid Technology • Key Features and Benefits • Quadruple Grating Architecture • Variable Band-Pass Selection • High-Performance, Easy-to-Use Filter System • Automated Z-Focusing • Dual Reagent Dispenser Option • Temperature Control System to 65°C • Take3™ Multi-Volume Plate with 2 µL microspots • Specifications and Summary

17. Take3™ Multi-Volume Multi-Sample Plate with 2 µL microspots • Sixteen 2 µL microspots • Enables low volume A260 nucleic acid quantification • dsDNA detection limit 2 ng/ µL • Can also be used for A280 protein quantification, as well as fluorescence or luminescence detection

18. Contents • Synergy H4 – Hybrid Technology • Key Features and Benefits • Quadruple Grating Architecture • Variable Band-Pass Selection • High-Performance, Easy-to-Use Filter System • Automated Z-Focusing • Dual Reagent Dispenser Option • Temperature Control System to 65°C • Take3™ Multi-Volume Plate with 2 µL microspots • Specifications and Summary

19. Summary: Versatility Redefined • Hybrid Technology: Monochromators and Filters • Quadruple Grating Optical Architecture • Variable Bandpass Selection • Dichroic Mirrors and Filters for High Performance • Dual Reagent Dispenser Option • Temperature Control System to 65°C • Take 3™ Multi-Volume Plate with 2 µL microspots

20. Visit www.biotek.com for more information