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Cloning Rab5WT and Rab5 Mutants to Study the Endocytic Pathway Involving TGF-  Receptor and Rin1

Cloning Rab5WT and Rab5 Mutants to Study the Endocytic Pathway Involving TGF-  Receptor and Rin1. Min Kang Colicelli Lab, UCLA. Growth factors. RTK. RTK. RAS. Adaptor. GEFs. GAPs. GTP. Rab5. Recycling endosome. Other Effectors. Early endosome. RAF. PI3K. RIN1.

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Cloning Rab5WT and Rab5 Mutants to Study the Endocytic Pathway Involving TGF-  Receptor and Rin1

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  1. Cloning Rab5WT and Rab5 Mutants to Study the Endocytic Pathway Involving TGF- Receptor and Rin1 Min Kang Colicelli Lab, UCLA

  2. Growth factors RTK RTK RAS Adaptor GEFs GAPs GTP Rab5 Recycling endosome Other Effectors Early endosome RAF PI3K RIN1 Early Endosome Trafficking is Required for RTK Down-Regulation MEK PIP3 ABL1/2 ERK AKT Late endosome/ lysosome Cell growth Cell survival Cell motility

  3. P P P P P P P P P P P P Clathrin- coated pit Lipid raft caveolae CAV1 P P RIN1 2 1 ABL RAB5 3 Early Endosome Caveolar vesicle RAB5 RIN1 Regulates TGF Signaling through Vesicles Trafficking SMAD2 SARA SMURF2 SMAD7 P SMAD2 TGFR downregulation TGF signaling

  4. Rab5 clones Rab5WT Rab5Q79L (DA) Rab5S34N(DN) Mlu Mlu EcoRI XhoI Nhe Nhe SalI M4-IRES-GFP M4-IRES-GFP M4-IRES-GFP

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