1 / 74

BIO 205 Microbiology with Lab

Denver School of Nursing – General Education Classes Lecture / Laboratory : Monday 10:00 am – 2:24pm Lecture: Tuesday 4:30pm – 6:30pm Instructor: Lisa Johansen, PhD Microbiology. BIO 205 Microbiology with Lab. Microbiology. When you see the word microbiology…

jason
Download Presentation

BIO 205 Microbiology with Lab

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Denver School of Nursing – General Education Classes Lecture / Laboratory : Monday 10:00 am – 2:24pm Lecture: Tuesday 4:30pm – 6:30pm Instructor: Lisa Johansen, PhD Microbiology BIO 205Microbiology with Lab

  2. Microbiology When you see the word microbiology… What do you think of??

  3. Microbiology What is your connection to microbiology?? Work? Home? Health?

  4. Chapter 1The Science of Microbiology

  5. The Scope of Microbiology • Six subgroups • Bacteria • Archaea • Algae • Fungi • Protozoa • Viruses • Helminths **

  6. Brief History of Microbiology • 1674 Leeuwenhoek: sees microorganisms • 1796 Jenner: vaccine for smallpox • 1847 Semmelweiss: cause of childbed fever • 1859 Pasteur: disproves spontaneous gen. • 1865 Lister: introduces antiseptic technique • 1876 Koch: pure culture on agar • 1892 Iwanowski: discovers viruses • 1894 Ehrlich: selective toxicity • 1929 Fleming: discovers penicillin • 1977 Woese: classifies archaea

  7. Animalcules • Made his own microscopes • Antony van Leeuwenhoek • Looked at everything he could • White matter from his teeth

  8. Edward Jenner and Immunity • Observation: • Dairymaids who had mild cowpox infections were protected from smallpox • Hypothesis • Cowpox infection provides protection against smallpox • Experiment • Inoculated boy with cowpox fluid and later challenged with smallpox fluid • Result • Boy did not get smallpox

  9. Childbed Fever • Wash your hands! • IgnazSemmelweis • Medical students were bring disease from the morgue to the women’s clinic

  10. Spontaneous Generation • Life is formed from inanimate objects • Fruit flies!!!

  11. Spontaneous Generation • Louis Pasteur • Used swan-neck flask • Boiled broth • Open to the air • No growth unless broth was washed into the curved neck

  12. Aseptic technique • Against infection via phenol • Joseph Lister • How good is the mouthwash though?

  13. Germ Theory of Disease • Koch’s Postulates • Microbes present in samples of diseased animal • Grow organism in pure culture • Inject healthy animal with cultured cells • Animal develops same disease

  14. Viruses are discovered • Smaller than bacteria - filterable • Dmitri Iwanowski and MartinusBeijerinick • Tobacco mosaic virus

  15. Selective Toxicity • Chemotherapy • Paul Ehrlich • Magic Bullet Theory

  16. Penicillin • The birth of antibiotics • Alexander Fleming • Bad lab techniques made him famous

  17. Archaea • Not just bacteria anymore • Carl Woese • Extremophiles

  18. Microbiology Today • Diagnostics • Treatments • Genomics • Epidemiology • Emerging diseases • Bioremediation • Environment micro / microbial ecology • Green fuels • Bioterrorism • Bioengineering • Agricultural microbiology • Industrial microbiology

  19. Six subgroups • Bacteria • Archaea • Algae • Fungi • Protozoa • Viruses • Helminths ** Chapter 10 meet the microbes!

  20. Classification systems and names Kingdom

  21. Writing names properly binomial nomenclature genus species Escherichia coli orEscherichia coli E. coli orE. coli

  22. bacteria = binomial nomenclature plus genus species strains E. coli K12 E. coli ML30 E. coli 0157:H7

  23. How we classify - methods - old

  24. How we classify - Dichotomous key - an oldie but goodie

  25. How we classify - methods - new molecular biology / genetics

  26. Molecular biology and identification

  27. The Prokaryotes - Ch. 11 Archaea Bacteria

  28. Prokaryotes: Homework • Chose 5 bacteria (total) from different 5 different phyla (Ch. 11) and describe: • habitat - where is it normally found? • shape (morphology - what does it look like under the microscope) • pathogenesis (does it cause disease? if so how?) • three interesting facts (not covered above) • think medical or environmental importance • unique features • include a picture of the organism • This must be a PowerPoint presentation. • This is part of your weekly presentation grade. • Due 1/14/13 @ beginning of class - on a thumb drive or email to me.

  29. Eukaryotes and Helminths and Arthropod vectors Chapter 12

  30. A few eukaryotes to know about: Fungi macroscopic microscopic

  31. Eukaryotic cells - Fungi heterotrophic saprobe

  32. Eukaryotic cells - Fungi yeast colonies mycelium spores

  33. A few eukaryotes to know about: Fungi

  34. Fungal diseases : thermal dimorphoism

  35. Mycoses = fungal infections thrush ringworm / tinea athletes foot / tinea Cryptococcus Aspergillus

  36. Good Fungi

  37. Good Fungi - antibiotic producers

  38. A few eukaryotes to know about: Algae

  39. A few eukaryotes to know about: Lichens

  40. A few eukaryotes to know about: Protozoa Amoeba Paramecium Giardia

  41. A few eukaryotes to know about: Protozoa: trypanosome

  42. A few eukaryotes to know about: Protozoa: Toxoplasma gondii

  43. A few eukaryotes to know about: Protozoa: Plasmodium

  44. A few eukaryotes to know about: Slime molds http://www.youtube.com/watch?v=bkVhLJLG7ug

  45. A few eukaryotes to know about: Helminths Intestinal Helminths

  46. Eukaryotes - Helminths Tapeworms Roundworms Flukes

  47. YOU and Helminths diseases

  48. Parasitic Helminths

  49. A few eukaryotes to know about: Arthropod vectors

  50. YOU and Arthropod vectors / diseases

More Related