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Scheme for BIOCHEMICAL REACTIONS. Urease test. -ve. +ve e.g. Proteus. Catalase Test. e.g. Streptococci. e.g Staphylococci. Catalase Test. e.g. Streptococci. e.g Staphylococci. Coagulase Test. Staphylococcus aureus. Coagulase negative staphylococci, e.g. S. epidermidis.
E N D
Urease test -ve +ve e.g. Proteus
Catalase Test e.g. Streptococci e.g Staphylococci
Catalase Test e.g. Streptococci e.g Staphylococci
Coagulase Test Staphylococcus aureus Coagulase negative staphylococci, e.g. S. epidermidis
Coagulase test Staphylococcus aureus Coagulase negative staphylococci, e.g. S. epidermidis
Oxidase test Oxidase positive e.g. Neisseria, Pseudomonas
Oxidase test Oxidase positive e.g. Neisseria, Pseudomonas
Oxidase test - ve + ve Oxidase positive e.g. Neisseria, Pseudomonas
Indole test + ve e.g. E.coli - ve e.g. Klebsiella
MR & VP tests MR test VP test +ve -ve
Citrate test Citrate positive Citrate negative
B.R. of E. coli Indole MR VP Citrate I M V C + + - - TSI
B.R. of Klebsiella VP I M V C - - + + TSI
Nagler’s Reaction(Clostridium perfringens) Antitoxin No antitoxin
Analytical Profile Index (API)Biochemical identification system
Analytical Profile Index (API) Biochemical identification system
Analytical Profile Index (API) Biochemical identification system
String Test (Positive in Vibriocholerae)
String Test (Positive in Vibriocholerae)
Tube agglutination test(Titre:1/320) 1/20 1/40 1/80 1/160 1/320 1/640 1/1280 - ve control TITRE
Brucella tube agglutination test showing prozone phenomenon (Titre:1/320) 1/20 1/40 1/80 1/160 1/320 1/640 1/1280 - ve control TITRE
Widal Test (Recent infection with S. Typhi)(Titre:1/320) 1/20 1/40 1/80 1/160 1/3201/640 1/1280 O C H C T C PA H C PB H
Widal Test (Old infection with S. Typhi) 1/20 1/40 1/80 1/160 1/3201/640 1/1280 O C H C T C PA H C PB H
Widal Test (Post vaccination) 1/20 1/40 1/80 1/160 1/320 1/640 1/1280 O C H C T C PA H H C PB
Single radial immunodiffusion 1 2 5 6 4 3
Elek’s Test Toxigenic C. diphtheriae Non-toxigenic C. diphtheriae
Western Blot for diagnosis of HIV
Tuberculin Skin Test (TST) Positive
Antibiotic sensitivity test(Disc diffusion method) 1 The organism is: Sensitive to 4, 5 & 6 Resistant to 1, 2 & 3 2 6 3 5 4
Antibiotic sensitivity test(Disc diffusion method) Sensitive to………… Resistant to………..
Antibiotic sensitivity test (Disc diffusion method) Sensitive to………… Resistant to………..
Antibiotic sensitivity test (Tube dilution method for MIC determination) MIC