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Streptavidin-HRP

Steroid sulfatase (STS). Streptavidin-HRP. Monoclonal STS antibody. J. B. C. 279 (2004) 44704. Biochemistry 2005, 44, 11669-11675. Tagging an ABP with biotin have two advantages: 1. To allows for the visualization of labeling events by avidin blotting

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Streptavidin-HRP

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  1. Steroid sulfatase (STS) Streptavidin-HRP Monoclonal STS antibody

  2. J. B. C. 279 (2004) 44704

  3. Biochemistry 2005, 44, 11669-11675

  4. Tagging an ABP with biotin have two advantages: 1. To allows for the visualization of labeling events • by avidin blotting 2. the MS characterization of labeling events by enrichment with avidin-conjugated beads. • biotin moieties remain a central tool for the target identification and certain gel-free profiling platforms, • protein blotting is a cumbersome and relatively low resolution method for the analysis of probe-labeled proteomes.

  5. Click Chemistry Azide Alkyne

  6. Proc. Nat. Acad. Sci. U.S.A., 104(8):2614 (2007)

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