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Streptavidin binding

A. O. H. N. N. H. 2. 3. 2. 4. 2. 5. 2. 1. 2. 2. H. 2. 0. 1. 9. N. 1. 4. 1. 7. 1. 5. 1. 2. 1. 0. 8. 6. 4. 2. 1. 3. S. S. 1. 8. 1. 6. 1. 1. 9. 7. 5. 3. 1. O. COO -. Streptavidin binding. Icmt substrate. B. 90. 80. 70. 60. 50.

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Streptavidin binding

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  1. A. O H N N H 2 3 2 4 2 5 2 1 2 2 H 2 0 1 9 N 1 4 1 7 1 5 1 2 1 0 8 6 4 2 1 3 S S 1 8 1 6 1 1 9 7 5 3 1 O COO- Streptavidin binding Icmt substrate B. 90 80 70 60 50 Icmt activity (pmol.hr-1.mg-1) 0.20  0.18 40 0.16 0.14 0.12 1/rate (pmol-1.hr.mg) 30 0.10   0.08  0.06  20  0.04     0.02 0.00 10 1/[BFC] (mM-1) 1 2 3 4 5 6 0 2 4 6 8 10 12 14 [BFC] (mM)

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