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Removal of low quality reads

Fig. S1. Software. Analytical workflow. Results. Sequence data. ABySS-tofastq. Removal of low quality reads. FASTX-toolkit. De novo assembly. Velvet & Oases. Removal of sequences shorter than 1,000 bp. Removal of highly similar sequences (similarity > 90%). Table 2. CD-HIT.

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Removal of low quality reads

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  1. Fig. S1 Software Analytical workflow Results Sequence data ABySS-tofastq Removal of low quality reads FASTX-toolkit De novo assembly Velvet & Oases Removal of sequences shorter than 1,000 bp Removal of highly similar sequences (similarity > 90%) Table 2 CD-HIT Blast against NCBI protein database BLASTX Back-mapping 50M reads to transcripts Bowtie Gene expression profiles Tables 3, 4, 5, 6 and S1; Fig. 1 Gene annotation Figs. 2a, b Blast2GO

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