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Transcription . Definitely not Transformation, Transduction or Translocation !. What is a Gene?. NOPE !. One Gene – One Enzyme One Gene – One Polypeptide One Gene – One RNA transcript A GENE IS A SEGMENT OF DNA THAT CODES FOR - - - - - - - - AN RNA TRANSCRIPT!!!!. NOPE !. YES !.

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  1. Transcription Definitely not Transformation, Transduction or Translocation!

  2. What is a Gene? NOPE ! • One Gene – One Enzyme • One Gene – One Polypeptide • One Gene – One RNA transcript • A GENE IS A SEGMENT OF DNA THAT CODES FOR - - - - - - - - AN RNA TRANSCRIPT!!!! NOPE ! YES !

  3. Which of the following will get translated? rRNA tRNA mRNA snRNA • A only • B only • C only • D only • A & B • B & C • C & D • A, B, & C • A, B, C, & D

  4. Which of the following are directly involved with translation? • A only • B only • C only • D only • A & B • B & C • C & D • A, B, & C • A, B, C, & D rRNA tRNA mRNA snRNA

  5. What best represents rRNA? • Red • Blue • Green • Brown

  6. What best represents tRNA? • Red • Blue • Green • Brown

  7. What best represents snRNA? • Red • Blue • Green • Brown

  8. Life is just an RNA SAMWICHH!

  9. Hey Check out this basic transcription animation! • http://www-class.unl.edu/biochem/gp2/m_biology/animation/gene/gene_a2.htm l

  10. What did the idiot scribe represent? • RNA primase • RNA polymerase • DNA polymerase • Reverse transcriptase • Helicase

  11. What is/are the substrate (s) for RNA polymerase? • ATP, GTP, UTP, CTP • ATP only • dATP, dGTP, dCTP, dTTP • ATP, GTP, CTP, TTP • Amino acids

  12. How many RNA polymerases are there? • THREE • RNA Polymerase I • Transcribes nucleolar genes into rRNA • RNA Polymerase II • Transcribes genes into mRNA (which eventually turns into a polypeptide) • RNA Polymerase III • Transcribes genes into tRNA and snRNA

  13. How does an RNA Polymerase “FIND” a gene to transcribe? • TRANSCRIPTION FACTORS FIND A TATA BOX ON THE PROMOTER REGION!!!!!!!!!!!!!!!!!!!!

  14. 3’ GGGCCCACGGAGCACAAAAA TTTTT ATATTTTT ATAGGGCCCGAGGTGTACGTCGAGCCC TTTACCATT GCGGGGAACACATTC TTATTTCCC GCGGACCC ACATCA TCAGGGG 5’ 5’ CCCGGGTGCCTCGTGTTTTTTAAAAATATAAAAATATCCCGGG CTC CACATGCAGCTCGGGAAATGGTAACGCCCC TTGTGTAAGAATAAAGGGCGCCTGGGTGTAGTAGTCCCC 3’ Blow UP! 3’ TACGTCGAGCCC TTTACCATT GCGGGGAACACATTC TTATTTCCC GCGGACCC ACATCA TCAGGGG 5’

  15. Check Out the Process in a MovEEE • http://vcell.ndsu.edu/animations/transcription/index.htm

  16. What did Sir Spliceolot accomplish? • Cut out different DNA sections called introns. • Cut out different RNA sections called exons. • Cut out different RNA sections called introns. • Cut out different DNA sections called exons.

  17. Why are introns called introns? They are INTERVENING noncoding sequences found within a gene!

  18. Why are exons called exons? They are coding sections of a gene that get EXPRESSED as a component of a protein!

  19. What really splices out the introns? • Cutterase • Dicerase • Spliceosome • snRNPase • Slicease

  20. What really is a SPLICEOSOME? small protein BIG PROTEIN snRNA snRNP SPLICEOSOME

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