Fig. 1

1. Fig. 1 a b 0 0.5 1 2 4 8 16 24 (h) - + + + + + + + CG200745 Acetyl-histone H3 β-actin c - (µM) 0.1 1 10 CG200745 SAHA Acetyl-histone H3 β-actin

2. Fig. 2 a 4 8 16 24 16 (h) 0 1 2 - + + + + + + CG200745 SAHA p53 Mdm2 p21Waf1/Cip1 β-actin b c

3. Fig. 3 a b * *p<0.05 *p<0.05 * * * * * RKO RKO RC10.1 RC10.1

4. Fig. 4 a 8hr+CHX+CG200745 8hr+CHX 5 0 5 10 20 40 60 0 10 20 40 60 (min) p53 β-actin b 8hr+CHX+CG200745 8hr+CHX

5. Fig. 5 a 0 1 2 4 8 16 24 (hr) 16 + + + + + - + IgG Input SAHA CG200745 IB: Ac-p53(K320) IB: Ac-p53(K373) IP: p53 IB: Ac-p53(K382) IB: p53 b p53 mutants Vector C p53 WT K320R K373R K382R p53 β-actin

6. Fig. 5 (continued) No treatment c CG200745 (10 μM) K320R K373R K382R C p53 WT p53 mutants

7. Fig. 6 No treatment CG200745 (10 μM) K320R K373R K382R C p53 WT p53 mutants

8. Supplementary Fig. 1 a K320R K373R K382R K320R K382R K373R K382R K320R K373R p53 WT vector K373R K382R C K320R p53 WT CG200745 p53 - - - - - - - - - - - Mdm2 + + + + + + + + + + + p21Waf1/Cip1 β-actin c b K320R K320R K373R K373R K382R K382R C p53 WT p53 mutants Vector Vector p53 mutants C p53 WT