Download
1 / 79
810 likes | 979 Views
Chem Picnic Saturday May 13 2-6 pm Fairhaven Park Sign up in the Chem office. "Cumulative Environmental Effects of Oil and Gas Activities on Alaska's North Slope". Dr. Gordon Orians, Emertius Professor of the University of Washington Monday May 15 at 6:30 pm in Fraser Hall, Room 4.
E N D
Chem Picnic Saturday May 13 2-6 pm Fairhaven Park Sign up in the Chem office
"Cumulative Environmental Effects of Oil and Gas Activities on Alaska's North Slope". Dr. Gordon Orians, Emertius Professor of the University of Washington Monday May 15 at 6:30 pm in Fraser Hall, Room 4.
Figure 14.18 VVP The two reactions of alcoholic fermentation. Page 604
Figure 14-20 VVP Reaction mechanism of pyruvate decarboxylase. Page 605
Fig 14-20 VVP
Fig 14-20 VVP
Figure 17-30 VVp. 451 in VVPThe reaction mechanism of alcohol dehydrogenase involves direct hydride transfer of the pro-R hydrogen of NADH to the re face of acetaldehyde.(p. 451 VVP) Page 606
Pyruvate Dehyrdogenase Reaction: Pyruvate + Coenzyme A + NAD+ Acetyl CoA + CO2 + NADH TCA Cycle : AcetylCo A + 3 NAD+ + FAD + GDP + Pi 2 CO2 + 3 NADH + FADH2 + GTP + CoA
Figure 16-1 Map of the major metabolic pathways in a typical cell. Page 550
Figure 21-3a Electron micrographs of the E. coli pyruvate dehydrogenase multienzyme complex. (a) The intact complex. (b) The dihydrolipoyl transacetylase (E2) “core” complex. Noncovalent assn. of prtoeins catalyzing sequential steps
Figure 21-4 Structural organization of the E. coli PDC. Even more complex in yeast and mammals! 12 dihydrolypoyl dehydrogenase (E3) (as dimers) 24 subunits Page 769 PDH: 24 Subunits (E1) (as dimers) E2 Dihydrolypoly transacetlyase core (trimers) a+b
Table 21-1 The Coenzymes and Prosthetic Groups of Pyruvate Dehydrogenase.
Figure 21-2 Chemical structure of acetyl-CoA. G = -31.5 kJ/mol Page 768
Figure 21-7 Interconversion of lipoamide and dihydrolipoamide. Page 771
Where have you seen this reaction before? Rxn 1: Pyruvate Decarboxylase! Electron sink nature of TPP delocalizes the negative charge on the carbanion intermediate
Rxn 2: Transfer of acetyl group to Lipoamide Attack of carbanion on disulfide followed by TPP elimination
Rxn 3: Transfer of acetyl group to CoA
Rxn 4: reoxidation of LA
Swings around among active sites
Figure 21-14 Catalytic reaction cycle of dihydrolipoyl dehydrogenase. Page 778
Figure 21-16 The reaction transferring an electron pair from dihydrolipoyl dehydrogenase’s (E3)redox-active disulfide in its reduced form to the enzyme’s bound flavin ring. FAD acts like an electron conduit between reduced disulfide and NAD+. Page 780
Figure 21-17a Factors controlling the activity of the PDC. (a) Product inhibition. Page 781 Products drive the red reactions backwards!
Figure 21-17b Factors controlling the activity of the PDC.(b) Covalent modification in the eukaryotic complex. Page 781
Fig 16-14 VVP p 486
Fig 16-2 VVP p 468
Figure 21-26 Amphibolic functions of the citric acid cycle. Page 793
Fig 16-5 VVP p 472
VVP p 480 H R R H + 2H+ + 2e- See Fig 17-10 VVP p 503
Fig 16-2 VVP p 468
Fig 16-9 VVP p 477
Fig 16-9 VVP p 477
Reaction occurs only at this bond Citrate is PROCHIRAL. VVP p 481
= from Acetyl-CoA VVP p 476
Fig 16-10 VVP p 477
VVP p 478 Mechanism: see Pyruvate DH
Fig 16-11 VVP p 479
In the absence of succinyl-CoA, the synthetase catalyzes the transfer of the -phosphate group from ATP to ADP, which suggests that the enzyme has a phospho-intermediate. VVP p 479
VVP p 480 H R R H + 2H+ + 2e- See Fig 17-10 VVP p 503
Fig 17-23 VVP p 521
Fig 14-16 VVP
