1 / 17

Chlorophyll and DO Analysis of a Duck Pond

Chlorophyll and DO Analysis of a Duck Pond. www.spcnp.org/ images/Male%20Wood%20Duck%201.jpg. Limnologists: Kyle Hewitt and Willie Adams. Eutrophication: The influx of nutrients into an aquatic ecosystem characterized by decreasing dissolved oxygen levels.

jimbo
Download Presentation

Chlorophyll and DO Analysis of a Duck Pond

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Chlorophyll and DO Analysis of a Duck Pond www.spcnp.org/ images/Male%20Wood%20Duck%201.jpg Limnologists: Kyle Hewitt and Willie Adams

  2. Eutrophication: The influx of nutrients into an aquatic ecosystem characterized by decreasing dissolved oxygen levels. www.umanitoba.ca/institutes/ fisheries/227_305.jpg Why is analysis of chlorophyll and DO levels in a Colorado Springs duck pond important? • Reveals the extent to which the pond has been eutrophied AND • Indicates the effect that this eutrophication has had on photosynthetic productivity at various locations in the pond

  3. Effect of Waterfowl Populations • As previous research has indicated water fowl populations have been known to cause pond eutrophication as a result of their significant contribution to the increase of nutrients (Hermanson 1999). http://www.entm.purdue.edu/wildlife/goose%20duck%20mix%20flock.JPG This has a direct effect on primary productivity

  4. Hypotheses • Chlorophyll levels in clear lakes are ~ 2 x 10-2 ug/ml (Krause-Jensen 1998). Levels at the duck pond will be significantly higher. • Furthermore, chlorophyll levels will be higher at the north end of the pond than the south as a result of increased sunlight and wind. • Finally Chlorophyll levels will be highest just below the suface (10cm)

  5. Methods • Field Equipment Used: • Sea King Row Boat (complete with paddles and life-jackets) • 15 500ml semi-transparent sample jars • Kemmerer • DO Meter • Cooler • Digital Camera

  6. “We’re gonna need a bigger boat!” Sample Locations: • Three sites in center • North Side Shore • South Side Shore

  7. Depth Variation This is a kemmerer! It is used to take water samples at different depths. This is Willie! He’s in a boat. Top Middle Bottom 10cm 0.75m 1.5m 18.7 º C 16.1 º C 14.2 º C

  8. North • Sunny • Windy • Water temperature 19.6º C • Large rocky shore • South • Shady • Calm • Water temperature 18.5º C • Gradual sandy Shore

  9. Later on, back in the lab • Lab Equipment Used • Vacuum Filter • Glass Fiber Filter Paper • Filtration Funnel • 90% Methanol • Petri dish • 90% Acetone • 1 cm cuvettes • 1 M HCl • Spectrophotometer • Procedure Day 1 • Filter 250ml of sample • Place filter in methanol

  10. 24 hours later. . . • Procedure Day 2 • Mix 4 ml methanol solution with 4 ml acetone • Take spectrophotometer readings at 665 and 750 nanometers. • Then add HCl and repeat. Chlorophyll conc. (ug/ml)=26.7*(665b-665a)

  11. Results Depth Bar graph showing values of chlorophyll concentration obtained at each location and depth (Mean +/- SD, n=3)

  12. Results cont. Depth Bar Graph showing values of dissolved oxygen at various locations and depths (mean +/- SD, N=3)

  13. More Results…… Scatter plot illustrating correlation between chlorophyll concentrations and DO for each sample

  14. Discussion Attack of the Koi! www.kaytee.com/images/ rotate/koi.jpg The little gosling that couldn’t….

  15. Concluding Statements • Pond has not yet entered a eutrophic state - - possibly mesotrophic. • Possible reasons for higher production on north side: • More Sunlight • More Nutrient Cycling (wind) • Landscape, Vegetation, Disturbance • Respiration is greater and photosynthesis lower at 1.5 meters • Further research . . . • “Chlorophyll? More like borophyll!!!” • -Billy Madison

  16. Literature Used: Arar EJ. (1997) Determination of Chlorophylls a and b and Identification of Other Pigments of Interest in Marine and Freshwater Algae Using High Performance Liquid Chromatography with Visible Wavelength Detection. From: National Exposure Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency. Baron JS. (2003) Sustaining Healthy Freshwater Ecosystems. Issues in Ecology. No. 10. Dineen CF. (1953) An Ecological Study of a Minnesota Pond. American Midland Naturalist, Vol. 50, No. 2. 349-376. Johannessen T, E Dahl (1996) Declines in Oxygen Concentrations Along the Norwegian Skagerrak Coast, 1927-1993: A Signal of Ecosystem Change Due to Eutrophication? Limnology and Oceanography. Vol. 41, No 4, 766-778. Krause-Jensen D et al. (1998) Light Attenuation and Photosynthesis of Aquatic Plant Communities. Limnology and Oceanography. Vol 43, no 3, 396-407. Latif Z et al. (2000) Evaluation of Water-Quality by Chlorophyll and Dissolved Oxygen. http://www.comsats.org.pk/latest/zahid_latif.pdf Steinman AD and GA Lamberti. (1996) Biomass and Pigments of Benthic Algae. Methods in Stream Ecology.

  17. Questions?? http://images.google.com/images?q=tbn:s0NZ8Aiu1xQJ:community.middlebury.edu/~dsanford/figaro/question.jpg http://www.infocopter.com/clipart/thumb-stupid.jpg http://images.google.com/images?q=tbn:C-cO7ntMxSIJ:www.amf.com/images/ball-question.gif http://qldkiteflyerssociety.com.au/fests/bondi_99/question.jpg

More Related