1 / 28

Enhancing Enzyme Kinetics and Bioreactor Operations: Uncompetitive Inhibition and Growth Modeling

Explore the effects of uncompetitive inhibition on enzyme-substrate interactions, rate law development, and bioreactor dynamics. Understand how intercepts and slopes change with varying inhibitor concentrations. Study enzyme kinetics, Monod equation, and cell growth phases in bioreactors. Dive into mass balance, yield coefficients, and substrate utilization for product formation and cell maintenance.

jirene
Download Presentation

Enhancing Enzyme Kinetics and Bioreactor Operations: Uncompetitive Inhibition and Growth Modeling

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Last Time 3/4/08

  2. Increase (I) concentration increase slope intercept remains unchanged.

  3. Uncompetitive Inhibition Inhibition only has affinity for enzyme-substrate complex Developing the rate law (1) (2)

  4. Adding (1) and (2) From (2)

  5. Total enzyme

  6. Slope remains the same but intercept changes as inhibitor concentration is increased

  7. Lecture16 Thursday 3/6/08 Enzyme Kinetics Noncompetive Inhibition Bioreactors Monod Equation Yield Coeficients Washout

  8. Bioreactors

  9. D=vo / V

  10. Noncompetitive, Bioreactors More uncompetitive inhibition Increasing I No I Only intercept changes Lineweaver-Burk Noncompetitive inhibition (Mixed) E + S E·S P E·I + S E·S·I Increasing I +I -I -I +I No I Both slope and intercept change

  11. Bioreactors Exponential growth phase Cells + substrate → product + more cells Cell growth can be modeled with enzyme equations since all cell reactors are enzyme catalyzed Lag phase stationary phase Death phase • Algorithm • Mass balance (later) • Rates sometimes Product concentration at which all metabolism ceases (93 g/L for wine production, n = ½) Substrate is used to form both products and cell maintenance (rate of substrate disappearance for maintenance

  12. 3) Stoichiometry – yield coefficients • Mass balance • Parameters: 2) Rates

More Related