1 / 28

Electrochemical Nanopatterning and Microsystems

Electrochemical Nanopatterning and Microsystems. Ioanis Katakis Department of Chemical Engineering, ATIC Technology Innovation Centre, Universitat Rovira i Virgili, Tarragona, Spain November 29, 2010 NANOJASP 2010, Barcelona. CONTROLLING RATES OF REACTION AND MASS TRANSPORT.

joie
Download Presentation

Electrochemical Nanopatterning and Microsystems

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Electrochemical Nanopatterning and Microsystems Ioanis Katakis Department of Chemical Engineering, ATIC Technology Innovation Centre, Universitat Rovira i Virgili, Tarragona, Spain November 29, 2010 NANOJASP 2010, Barcelona

  2. CONTROLLING RATES OF REACTION AND MASS TRANSPORT HOLDING EVERYTHING TOGETHER MODULATING ACTIVITIES THE TRANSDUCTION CHEMISTRY THE BIOMOLECULE(S) THE TRANSDUCER DEFINING TERMS • The generic bioelectronic element

  3. MODULAR IMMOBILISATION... • Each module can be any of the components. You aim at flexibility and control of outcome. • Narváez et al. Biosens Bioelectr 15:43-52 (2000) • Narváez et al. J Electroanal Chem 430:227-33 (1997)

  4. …TO CONTROL PROPERTIES Fructose modular electrodes • Rational manipulation of bioregeneration kinetics

  5. Substrate NAD(P)+ Mediatorred Dehydrogenase Electrode e- Product NAD(P)H Mediatorox APPLY JUDICIOUSLY MOLECULAR ENGINEERING SUPRAMOLECULAR ARCHITECTURES FOR COMPLEX BIOSENSING, AMPLIFICATION, AND CATALYSIS TASKS • Popescu et al. J Electroanal Chem 464:208-14 (1999)

  6. BUT WHAT ABOUT PATTERNING? • CONTACT AND CONTACT-LESS SPOTTING WELL DEVELOPED FOR 100+ mm RESOLUTION • BIOMOLECULE PHOTOLITHOGRAPHY FOR HIGH DENSITY APPLICATIONS WHAT IF WE COULD USE ELECTROCHEMICALLY-DRIVEN METHODS FOR BOTH PATTERNING AND DETECTION?

  7. Electrostatic forces + - - - - + - - + - - - s Dative binding Adsorptionphenomena “NANO” ENGINEERING SPATIAL INTELLIGENCE • Colloidal gold: a versatile nano/module

  8. conjugation oligonucleotide gold colloid deposition colloidal gold-oligonucleotide selective deposition PATTERNING BIOLOGICAL PROPERTIES NANOCOLLOID SYNTHESIS AND MODIFICATION FOR NANOPATTERNING AND ARTIFICIAL INTELLIGENCE • Campàs & Katakis Int J Env Anal (2004), PCT/EP2003/000262 (2002) • Campàs & Katakis Sens. & Actuators B (2006)

  9. e- Electrochemistry on SPE ARE THEY FUNCTIONAL? • Concept works but high exists non-specific adsorption

  10. GOx GOx GOx GOx GOx MOLECULAR ENGINEERING OF “NANO”... • Adding properties (transduction) to nanopatterns

  11. PEG PEG PEG PEG Glucose e- H2O2 e- SH SH SH SH Au Au Amperometric detection of HRP (e1) Amperometric detection of GOx (e2) e2 -1.2V) selective desorption Non-specific adsorption was detected on the second electrode 5% of non-specific response was detected from the first electrode The deprotection of the second electrode avoids the non-specific adsorption detected. There is only a 3,4% of non-specific response from the second electrode Thioctic acid SAM …AND IMPROVING SELECTIVITY • Amperometric detection of electrodeposited biomolecules e1 (-1.2V) desorption of Thioctic acid e2 (+1.2V) deposition of Gox-Os-Au e1 (+0.8V) selective adsorption of HRP-Os-Au

  12. O O O O O O O O O O O O O O O O O O O O O O O O H O O O O O O O O H H H H H H H O O O O O O O O O O O O O O O O +700mV O O O H Au Au S S S S S S S S S S S S S S S S S S S S ONE STEP FURTHER: PATTERNS AT MOLECULAR LEVEL

  13. Peak around +0.6V DOES IT WORK? Electrochemical deprotection was nearly complete within one scan

  14. IS IT SELECTIVE? • EQCM data shows hope (but still 30% non specificity)

  15. AND YET ANOTHER METHOD OF PATTERNING • First laser exposure • Resist coating • First biomolecule coating • Second laser exposure • Second biomolecule coating • Polyelectrolyte blocking Laser ablation or lithography work equally well

  16. SOX 2h. POS+SOX GOX GOX 2h. POS+GOX BIOPHOTOLITHGRAPHY: CATALYSIS(1) • Layer optimisation: GOX(first layer) SAOX (second layer) • After third layer of polyelectrolyte the response for enzyme decrease 30%.

  17. Gox and SAOx response with Glucose SOX 2h. POS+SOX GOX SAOx GOX GOX 2h. POS+GOX BIOPHOTOLITHGRAPHY: CATALYSIS(1) • Layer optimisation: GOX(first layer) SAOX (second layer) GOX (Max. Response 856A) SOX (Max. Response 10 nA)

  18. GOX Gox and SAOx response with Sarcosine 2h. POS+GOX GOx SAOx SOX SOX 2h. POS+SOX BIOPHOTOLITHGRAPHY: CATALYSIS(2) • Layer optimisation: SAOx (first layer) GOX (second layer) GOX (Max. Response 0A) No crosstalk. SOX (Max. Response 101 nA)

  19. H2O2 e- Au Au HRP HRP Also in CE higher signal was obtained from the electrode with wild probe (50nA), while 0nA was obtained from electrode where mutated probe was immobilised IDE: Higher signal was obtained from the electrode with wild probe (1.7A), 0.4A was obtained from the mutated probe and 0.3 A from the control without target. 1st electrode (Sample) 2nd electrode (Control) BIOPHOTOLITHGRAPHY: HYBRIDISATION Incubation of both electrode with streptavidine-HRP Immobilisation of biotinylated wild capture probe in e1 Incubation of both electrode with the biotinylated target Amperometric detection of both electrodes Incubation of e1 with redox polymer and streptavidine Immobilisaion of biotinylated mutated capture probe in e2 Incubation of e2 with redox polymer and streptavidine BSA blocking in e1 Deprotection of e1 with UV BSA blocking in e2 Deprotection of e2 with UV

  20. H2O2 e- Au Au HRP Also in CE there is a lower response from the control, nevertherless the signal is lower. IDE: lower signal was obtained comparing with DNA, however as in DNA wafers the control was lower (2nA) than the sample (40nA) 1st electrode (Sample) 2nd electrode (Control) BIOPHOTOLITHGRAPHY: MOLECULAR RECOGNITION(1) • HCG amperometric detection through sandwich assay Incubation with redox polymer and anti-HCG in e2 Incubation with redox polymer and anti-HCG in e1 Incubation of HCG target and biotinylated anti-HCG in e1 e1 deprotection Incubation of biotinylated anti-HCG in e2 (control) Incubation of both electrodes with streptavidine-HRP BSA blocking in e2 Amperometric detection of HRP e2 deprotection BSA blocking in e1

  21. H2O2 e- Au Au HRP A competition assay was carried out to detect T4. 94.2nA was obtained from the control and 47.3nA from the sample 1st electrode (Control) 2nd electrode (Sample) BIOPHOTOLITHGRAPHY: MOLECULAR RECOGNITION(2) • T4 amperometric detection through competition assay Incubation of both electrodes with anti-Rabbit Igg-HRP Incubation with redox polymer and BSA-T4 in e2 Incubation with redox polymer and BSA-T4 in e1 Incubation with anti-T4 in e1 (Control) Incubation with T4 and anti-T4 in e2 Amperometric detection of HRP e2 deprotection BSA blocking in e2 BSA blocking in e1 e1 deprotection

  22. SH SH SH H2O2 SH SH SH SH HRP e- A difference of 1,5µA between sample SH and blank and a limit of detection of 6.31fmoles was obtained MODULATING ACTIVITY: RECOGNITION TO SENSING

  23. H2O2 H2O2 e- e- HRP - 57% and 23% of signal displaced in colourimetric and electrochemical displacement - Fast response: 2 minutes in electrochemical displacement AND FROM SENSING TO FACILE SENSING

  24. e- Peroxidase uses H2O2 and consumes electrons at E2 Glucose oxidase produces H2O2 Glucose oxidase produces electrons at E1 e- + + + + + + + + + + + + - - - + + + + + + - - - - - - - - - Os+ Os+ - - - Os+ Os+ - - - Os+ Os+ Os+ Os+ Os+ Os+ Os+ Os+ • Pescador et al Langmuir (2008) INTEGRATING TECHNOLOGIES FOR MORE FUNCTIONS TOWARDS THE ULTIMATE NANOMACHINE(?): SELF POWERED, SELF PROPELLED, SELF PROPAGATING }N2 }N1

  25. Mata at al Electroch. Acta (2009) ELECTRODEPOSITION AS PART OF OPERATION Using same principles for versatile microsystem operation

  26. NCSR DEMOKRITOS • Panagiotis Argitis Universitat Rovira i Vrigili • Mònica Campàs • Mònica Mir • Srujan Dondapati • Pablo Lozano THE TEAM

  27. THE MONEY • Our work is financed by: • MICROPROTEIN (Patterning and arraying) • HEALTHY AIMS (Fuel cells) • CELSITIVE (Pathogen Detection) • CIDEM and our Clients • URV

  28. THANK YOU FOR YOUR ATTENTION

More Related