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Distinct Features of NLC

Distinct Features of NLC. Blood Monocytes. Nurselike Cells. Relative cell number. CLL B cells. Normal B cells. BAFF. Nurselike Cells Express BAFF. Immunofluorescence Microscopy Of NLC and CLL B cells. Red = BAFF Green = CD19 (CLL cells). Gray histograms = BAFF

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Distinct Features of NLC

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  1. Distinct Features of NLC

  2. Blood Monocytes Nurselike Cells Relative cell number CLL B cells Normal B cells BAFF Nurselike Cells Express BAFF Immunofluorescence Microscopy Of NLC and CLL B cells Red = BAFF Green = CD19 (CLL cells) Gray histograms = BAFF Clear histograms = Control

  3. Nurselike Cells Express APRIL Real-time RT-PCR Immunofluorescence Microscopy Of NLC and CLL B cells 3000 ** 2500 2000 APRIL mRNA (Units) 1500 1000 500 0 CLL B Cells Monocytes Nurselike Cells Normal B Cells Immunoblot Analysis Normal B Cells CLL B Cells 2 Monocytes CLL B Cells 1 NLC APRIL Green = APRIL Red = CD19 (CLL cells) -actin

  4. Endo, T, et al. Blood 109:703-710, 2007

  5. BAFF APRIL LTR BAFF-R BCMA TACI BAFF-R ?

  6. BAFF/APRIL Activation of NFkB Cytoplasm Nucleus rhAPRIL rhAPRIL Medium Medium rhBAFF rhBAFF p100 p100 p52 p52 p65 p65 b-actin SP-1 p50 p50 p50 p50 p50 p50 p50 p65 p65 p65 p65 p65 p65 p65 p50-p65 p50-p50 Medium rhBAFF rhBAFF + anti-BR3 rhBAFF + BCMA-Fc rhBAFF + BR3-Fc rhAPRIL rhAPRIL + BCMA-Fc NF-Y

  7. BAFF-R BAFF-R BCMA LTR TACI ? BAFF APRIL

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