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Signal Transduction Pathways

Signal Transduction Pathways. Signaling Pathways Control Many Cellular Processes. Cell Shape. Cell Polarity. Cell Cycle. Development ….. and Disease. Signals. Stop!. ……….Pathway OFF. Go Faster?. ….…Basal. Go. ……..Pathway ON. Stimulus Receptor PAK/G-protein MAP Kinase Cascade

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Signal Transduction Pathways

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  1. Signal Transduction Pathways

  2. Signaling Pathways Control Many Cellular Processes Cell Shape Cell Polarity Cell Cycle Development ….. and Disease

  3. Signals Stop! ……….Pathway OFF Go Faster? ….…Basal Go. ……..Pathway ON

  4. Stimulus Receptor PAK/G-protein MAP Kinase Cascade Txn Factors Generic MAP Kinase Pathway

  5. Yeast Genetics Genomic Screens Genomics DNA Microarrays Cell Biology GFP Collection Biochemistry TAP Tag Collection Budding Yeast is a Model Organism Genetics and High-throughput Technologies

  6. Filamentous Growth (FG) in Yeast Saccharomyces cerevisiae FG is required for fungal pathogenesis.

  7. HOGP Sho1 Ste20/Cdc42 Ste11 Pbs2 Hog1 Hot1 FGP Sho1 + ? Ste20/Cdc42 Ste11 Ste7 Kss1 Ste12/Tec1 FGP ? Ste20/Cdc42 Ste11 Ste7 Kss1 Ste12/Tec1 PRP Ste2/3/4/18 Ste20/Cdc42 Ste11 Ste7 Fus3 Ste12 Ste5 MAPK Pathways Share Factors

  8. Genome-Wide Genetic Screen Mutants defective for agar-invasion (FG)

  9. DNA Microarray Analysis UP NO CHANGE DOWN

  10. MSB2 was identified in both screens. Comparison of Genomic Data

  11. Mucin Domain Sho1 Cdc42 Scaffold at the Head of the FG Pathway Msb2

  12. FUS1-lacZ Mucin domain is inhibitory. Role of Mucin Domain in Signaling N…(698)S Q V S D T S V S Y T T S S S S V S Q V S D T P V S Y T T S S S S V S Q V S D T P V S Y T T S S S S V S Q V S D T P V S Y T T S S S S V S Q V S D T P V S Y T T S S S S V S Q V S D T S V PS T S S S S S V S Q V S D T P V PS T S S S S S V S Q (818) …C

  13. Hyperactivity Go, Go, Go!

  14. Questions about the FG pathway What is Msb2 sensing? How are general factors specified by Msb2? In what other ways is specificity achieved?

  15. Negative Regulation Stop!

  16. HOGP Sho1 Ste20/Cdc42 Ste11 Pbs2 Hog1 Hot1 FGP Sho1 + Msb2 Ste20/Cdc42 Ste11 Ste7 Kss1 Ste12/Tec1 Novel Negative Regulator: a Calmodulin Kinase Rck2

  17. Questions about Rck2 Where does the kinase act? How does P~lation by Rck2 cause pathway shut-off? Is the response specific?

  18. Rotate in Paul Cullen’s Lab? Sure!

  19. Training in Paul Cullen’s Lab Question-based approach Learn many different techniques (Genomics) Working with yeast is Fun!

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