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ATB: OPEN LOGBOOK TO BEAN DEMO ENTRY AGENDA: NO HW

ATB: OPEN LOGBOOK TO BEAN DEMO ENTRY AGENDA: NO HW. ATP. Mitochondria. H 2 O. O 2. CO 2. glucose C 6 H 12 O 6. ( starch ). Chloroplasts. Sun.

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ATB: OPEN LOGBOOK TO BEAN DEMO ENTRY AGENDA: NO HW

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  1. ATB: OPEN LOGBOOK TO BEAN DEMO ENTRY • AGENDA: NO HW

  2. ATP Mitochondria H2O O2 CO2 glucose C6H12O6 (starch) Chloroplasts Sun In the network diagram on the left-we see that excess glucose is stored as starch. We also see that starch is converted back to glucose. We know that cellular respiration requires glucose—not starch. How could we get evidence that a bean seed 1) contains stored starch and 2) performs cellular respiration when provided the right conditions (moisture) for germination

  3. DAY 1 Beans in Iodine Solution—testing for starch and glucose Beans in M.B. Solution—testing for oxygen Beans in BtB Solution—testing for CO2 = Bean Seed Glucose test strip—negative test for glucose

  4. DAY 2 Beans in Iodine Solution—testing for starch and glucose Beans in M.B. Solution—testing for oxygen Beans in BtB Solution—testing for CO2 = Bean Seed Glucose test strip—positive test for glucose

  5. Lactose Intolerance and Lactase (Why lactase does a body good)

  6. Questions for Reading • Explain why lactase is essential for the proper digestion of milk. • Based on what you know about enzymes, do you think the lactase molecule can be reused? Why or why not? • Based on what you know about enzymes, do you think lactase can break down other disaccharides besides lactose? Why or why not? • Imagine you are a doctor or pharmacist: How would you treat a patient who likes to drink milk but has lactose intolerance?

  7. Take out your questions from your reading on lactose intolerance and lactase • Answer on your warm-up paper: • What does lactase do? • How could you measure the activity of lactase in an experiment? • What happens to the activity of an enzyme after it is boiled?

  8. Lactose is a disaccharide: It is formed from the combination of glucose and galactose. This reaction can be reversed by the enzyme lactase. Lactose Lactose

  9. Lactase lab Pre-lab questions: • What does lactase do? • How can the activity of lactase be measured in an experiment? • What controls would you need for an experiment to test the activity of lactase?

  10. ATB: OPEN LOGBOOK TO “LACTASE LAB” • LACTASE LAB WRITE UP DUE WEDNESDAY-ON YOUR OWN PAPER

  11. Lactase Lab • We will conduct a lab to determine how boiling affects the activity of an enzyme.

  12. Testable question: How does boiling affect the activity of an enzyme? • Materials: 4 test tubes, test tube rack, milk (lactose), lactase solution, boiled lactase solution, 4 glucose test strips, pipettes

  13. Lactase Lab Illustration: Glucose Test Strip Key: Pink/Light Purple-No Glucose Darker Purple/Blue—Glucose present milk + lactase milk + boiled lactase milk lactase

  14. Data tables V. Observations/Data Collection Analysis / Data Presentation

  15. Post-lab questions • What color on the test strip means that glucose is present? • What color on the test strip means that glucose is not present? • From your results, how do you know that milk doesn’t have glucose in it? • Glucose was present when lactase and milk were mixed. From your results, how do you know that the lactase solution didn’t have glucose in it before it was mixed?

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