10 likes | 115 Views
MICR 300 Section 03 Experiment 14 Plasmid Isolation, Restriction Digest, and Minigel Electrophoresis 11.05.07. 1 2 3 4 5 6 7 8. 1 2 3 4 5 6 7 8. Table 1. Table 2. 1 2 3 4 5 6 7 8. 8 7 6 5 4 3 2 1. Table 3. Table 4. 1: plasmid + water
E N D
MICR 300 Section 03 Experiment 14 Plasmid Isolation, Restriction Digest, and Minigel Electrophoresis 11.05.07 1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8 Table 1 Table 2 1 2 3 4 5 6 7 8 8 7 6 5 4 3 2 1 Table 3 Table 4 1: plasmid + water 2: plasmid + EcoR1 (expect 2 cuts) 3: plasmid + DraI (expect multiple cuts) 4: Ladder 5: Positive control Plasmid + one enzyme (Table1,3: DraI, Table 2,4: ecoR1) 6: plasmid + water 7: plasmid + EcoR1 8: plasmid + DraI