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Differentiated (4 d). Undifferentiated. Supplemental Figure S1. Supplemental Figure S2. TRE. GC box. Supplemental Figure S3. A. a. b. c. d. e. 8 d. N5. N4. N3. N2. N1. 0 d. ApaI. XhoI. PstI. SmaI. ApaI. f. g. h. i. PstI. ApaLI. PstI. SpeI. B. a/b. Xho1. a/c. N5.
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Differentiated (4 d) Undifferentiated Supplemental Figure S1
TRE GC box Supplemental Figure S3
A a b c d e 8 d N5 N4 N3 N2 N1 0 d ApaI XhoI PstI SmaI ApaI f g h i PstI ApaLI PstI SpeI B a/b Xho1 a/c N5 a/d N4 Pst1 N3 a/e Sma1 f/g N2 Spe1 f/h N1 ApaL1 f/i 0 2 4 6 8 0 2 4 6 8 Mock Mock Diff. (d) Diff. (d) Supplemental Figure 4
A Diff. (days) N4 N5 8 N3 N2 N1 N-1 6 4 2 0 e a n g o b k h f p c l i q d m -1.0 kb -0.5 kb + 1 XhoI PstI SmaI SpeI ApaLI TRE GC boxes B H2O 0 2 4 6 8 H2O 0 2 4 6 8 Diff. (d) a j b k N5 j c N2 l d m e N4 n f N1 o g p h N3 N-1 q i Supplemental Figure S5
Table S1. Primer pairs for RT-PCR Table S2. PCR primers for ChIP analysis S and AS represent for sense and antisense sequence, respectively.
Table S3. PCR primers for restriction enzyme (RE) accessibility test in Supplemental Figure S2 S and AS represent for sense and antisense sequence, respectively.
Table S4. PCR primers for mononucleosome survey in Supplemental Figure S3