1 / 21

MICB 405 Bioinformatics Mini-Lab #2 - BLAST

MICB 405 Bioinformatics Mini-Lab #2 - BLAST. Dr. Joanne Fox joanne@msl.ubc.ca. Objectives. By the end of today’s tutorial: You will use the web interface at the NCBI to run a BLAST search.

joy
Download Presentation

MICB 405 Bioinformatics Mini-Lab #2 - BLAST

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. MICB 405 BioinformaticsMini-Lab #2 - BLAST Dr. Joanne Fox joanne@msl.ubc.ca

  2. Objectives • By the end of today’s tutorial: • You will use the web interface at the NCBI to run a BLAST search. • You will investigate the graphical view, the BLAST “hits” list, and the pairwise alignments for the results of this BLAST search. • You will view the taxonomy reports for this BLAST result.

  3. search Search Entrez for NP_644805.1

  4. Retrieve FASTA sequence FASTA

  5. Copy (and Paste) Sequence

  6. A graphical view of your BLAST results:

  7. The BLAST hit list

  8. BLAST local pairwise alignment

  9. Distance tree of results

  10. BLAST Tree View Widget Stat 5A Stat 5B

  11. Taxonomy reports

  12. Sorting BLAST results by Taxonomy

  13. Formatting options

  14. Limit Results by Organism

  15. Links • NCBI BLAST page http://www.ncbi.nlm.nih.gov/BLAST/ • The NCBI Query Tutorial http://www.ncbi.nlm.nih.gov/Education/BLASTinfo/query_tutorial.html • The NCBI BLAST Tutorial http://www.ncbi.nlm.nih.gov/Education/BLASTinfo/tut1.html • The NCBI BLAST Program Selection Guide. http://www.ncbi.nlm.nih.gov/blast/producttable.shtml

More Related