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Experiment Eight: Pharmacological Neuromodulation

Experiment Eight: Pharmacological Neuromodulation. Pharmacological neuromodulation. Test effect of neuroactive compounds on neurons Only certain drugs affect neurons without danger Nicotine Glutamate Ethanol. wikipedia. Nicotine. Acetylcholine receptor agonist

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Experiment Eight: Pharmacological Neuromodulation

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  1. Experiment Eight: Pharmacological Neuromodulation

  2. Pharmacological neuromodulation • Test effect of neuroactive compounds on neurons • Only certain drugs affect neurons without danger • Nicotine • Glutamate • Ethanol

  3. wikipedia

  4. Nicotine • Acetylcholine receptor agonist • Amplifies effect of Ach binding to receptors in synapses • Causes a neuron to fire more

  5. Monosodium glutamate • Neurotransmitter • Dissolved in water, glutamate ions released • Over 80% of synapses in brain use glutamate as excitatory neurotransmitter

  6. Ethanol • GABA receptor agonist • Unlike Ach receptor, inhibits action potentials • Causes a neuron to fire more slowly

  7. Exercise 1: preparing neuroactive solutions • Saline: combine 1.5 g table salt and 1.25 g baking soda in 250 mL distilled water • Nicotine: dissolve tobacco from cigarette in saline • Glutamate: dissolve MSG in saline • Ethyl alcohol: mix 196 mL saline with 4 mL ethanol (2% EtOh) • Mix 12.5 mL 2% EtOh with 87.5 mL saline (0.25% EtOh)

  8. Exercise 2: Cricket Cercal Ganglion Preparation • Place crickets in freezer for 5 minutes to anesthetize • Decapitate cricket at junction between head and thorax • Insert electrode through abdomen into cercal ganglion

  9. Exercise 3: Control Cercal Response • Stimulate cerci by blowing from right or left side of cricket • Record on Audacity or mobile device • Record time and duration of stimulus (blowing) and side the stimulus was delivered (right or left)

  10. Exercise 4: Testing Neuroactive Compounds • Record with Audacity • Inject a few drops of compound in abdomen • Stimulate cerci as in Ex. 3 • Amplify and sketch traces of stimulations on chart • Wash recording electrode with saline • Repeat for nicotine, glutamate, and both ethanol solutions

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