1 / 16

Experiment eight Medical Mycology

Experiment eight Medical Mycology. ---Fungi. Introduction Fungi are eukaryotic microorganisms that possess a cell wall and a nucleus enclosed by a nuclear membrane, and they do not contain chlorophyll. Medically important fungi. Includes 4 phyla

pelham
Download Presentation

Experiment eight Medical Mycology

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Experiment eight Medical Mycology ---Fungi

  2. Introduction • Fungi are eukaryotic microorganisms that possess a cell wall and a nucleus enclosed by a nuclear membrane, and they do not contain chlorophyll.

  3. Medically important fungi Includes 4 phyla • Ascomycota（子囊菌门）- Sexual reproduction in a sack called an ascus with the production of ascopspores. • Basidiomycota（担子菌门）-Sexual reproduction in a sack called a basidium with the production of basidiospores. • Zygomycota（接合菌门）- sexual reproduction by gametes and asexual reproduction with the formation of zygospores. • Mitosporic Fungi（Fungi Imperfecti，半知菌门）- no recognizable form of sexual reproduction. Includes most pathogenic fungi.

  4. I. Morphology A. Size: • several to tens times larger than bacteria B. Two basic morphological forms • Yeast: monocellular fungi • Yeast form fungi • Yeast-like form fungi (Candida albicans) • Mold: multicellular fungi • Hypha + spore

  5. Yeast budding • Monocellular fungi • Round or oval • Reproduce by budding/fission • Pseudohypha Pseudohypha

  6. Mold • Multicellular fungi • Hypha • Spore • Fungi identification

  7. Hypha

  8. hypha spore

  9. Hypha • branching, threadlike filaments of fungi that elongate at their tips • septate hypha \ coenocytic hypha • 3 kinds of hypha • Vegetative hypha • Aerial hypha • Reproductive hypha: aerial hypha that produce spores Mycelium • The complex of a mass of hypha

  10. II. Culture • Culture medium: Sabouraud’s agar (4% glucose, 1% peptide, 2% agar) • 22~28℃, pH 4~6; • High moisture and O2; • Fungal colony a. Yeast colony, e.g., Cryptococcus neoformans. b. Yeast-like colony, e.g., Candida albicans. c. Filamentous colony, e.g., mold

  11. Yeast colony Round, pasty/mucoidcreamy, opaque colonies similar to bacterial colony

  12. Yeast-like colony Candida albicans

  13. Filamentous colony Mold colony Penicillium colony

  14. Cryptococcus neoformans • Round, capsule, 3~5 m • 4~7 m in diameter • blood agar and Sabouraud’s agar 25℃ & 37℃ nonpathogenic cryptococcus cannot grow at 37℃ • yeast form colony

  15. Examine fungi of the skin • Direct examination Material: scissors , sterile tweezers ,slides, lid slides,10%KOH. Procedure: 1.Make a smear: take some skin tissues from the foot on the slide. Drop 1 drop of 10%KOH solution to the skin. Wait for a while. Put the lid slide on the skin tissues and heat slightly. 2.Direct examine with the microscope.

  16. Culture: Culture medium: Sabouraud medium Method: streak plate 22~28℃,incubate 1~7 days Detection of colony Gram-stain

More Related