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Supplementary figure 1 PCR analysis of the PAX3/7-FOXO1 transcript in all cell lines. Expression of the PAX3-FOXO1 fusion transcript was confirmed in the ARMS cell lines RMS, RH41, RH30 & RH3. Expression of the PAX7-FOXO1 fusion transcript was confirmed in the ARMS cell line RMZ-RC2. The ARMS cell line RH18, the ERMS cell line RD and both primary myoblast cell typeswere negative for the PAX3-FOXO1fusion transcript. Myo- blasts RMZ (Bio- RH3 -RC2 Heart) RMZ-RC2 PAX7-FOXO1 fusion transcript PAX3-FOXO1 fusion transcript