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CC-NBS-LRR (CNL). Transposable element. F-box. Other gene. Truncated gene. Khipu tandem repeat. Unknown physical distance. Putative location of the minor knob. Location of the major knob. K. K. 43. CNL-B12 (r). 44. CNL-B13 (r). 45. CNL-B14* (r). 46. CNL-B15 (r). 47.
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CC-NBS-LRR (CNL) Transposable element F-box Other gene Truncated gene Khipu tandem repeat Unknown physical distance Putative location of the minor knob Location of the major knob K K 43 CNL-B12 (r) 44 CNL-B13 (r) 45 CNL-B14* (r) 46 CNL-B15 (r) 47 CNL-B16 (r) David_Supplemental Figure S1 Phaseolus vulgaris B4 locus Genetic map B4 locus GP 4 8 CNL-B21 Sub-cluster D (81/83/85) 9 CNL-B22 10 CNL-B23 K 11 CNL-B1* (d) 12 CNL-B2* (d) 13 CNL-B3 (d) 14 CNL-B4* (d) 26.4 cM 15 CNL-B5* (d) 16 CNL-B6* (d) CNL-B7 (d) 17 Sub-cluster C (83.5/87.5/95) 18 CNL-B8 (d) A 8 khipu tandem units; 4,288 bp (d) B 3 khipu tandem units; 1,611 bp (d) 19 CNL-B9 (d) 20 CNL-B10*(d) 21 CNL-B11 (d) C 29 khipu tandem units; 15,246 bp (r) D 3 khipu tandem units; 1,585 bp (d) GP 3 E 4 khipu tandem units; 2,135 bp (r) 0.7 cM GP 2 F 5 khipu tandem units; 2,605 bp (r) Sub-cluster B (75/85/90) 48 CNL-B17 (r) 7 khipu tandem units; 3,586 bp (r) G 22 khipu tandem units; 11,748 bp (r) H 2 cM 49 CNL-B18 (r) CNL-B26* (d) 2 CNL-B27 (d) 3 CNL-B28 (d) 4 1 khipu unit; 502 bp (d) I 2 khipu tandem units; 1,121 bp (d) J Sub-cluster A (85/89.5/94) K 3 khipu tandem units; 1,625 bp (r) GP 1 10 CNL-B29 (d) 13 CNL-B30* (d) L 5 khipu tandem units; 2,584 bp (d) Telomere 50 kb K
Supplemental Figure S1. Simplified representation of the 650 kb sequence corresponding to the Phaseolus vulgaris B4 resistance locus showing details concerning khipu blocks. Twenty-six CNL (blue arrows) are regrouped in four sub-clusters named A to D. Two gaps are indicated by dotted lines. The orientation of predicted genes is indicated by colored arrows. Khipu tandem repeats (A – L) are in purple, with a purple arrow on the left. Numbers of khipu units from 1 in “I” to 29 in “C” and the size of the corresponding region are indicated in bracket after the name of the considered khipu tandem repeat. Khipu tandem repeat are mostly found in sub-cluster A to C. Genetic positions are shown on the right. Orientation of khipu tandem repeats and CNL is indicated by letters “d” or “r” in bracket for direct and reverse, respectively. Markers derived from the BAC clones described in David et al. (2008) are presented to the right of their corresponding genetic position. BAC clones FZ-E9 and 48-B10 have been mapped at GP 4 and 1, respectively. Orientations of these two BAC clones are unknown, so arbitrary orientations were chosen for this schematic representation. The % of nucleotide identity (minimum/average/maximum)among the CNL of a considered sub-cluster are presented in brackets under the name of each sub-cluster.