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Concentration Matters

10 ng of sample (note: we request 50 ng of DNA in 10ul and run 5ul on the 3130xl). Concentration Matters. Note the areas of low resolution. 20 ng of sample (note: we request 50 ng of DNA in 10ul and run 5ul on the 3130xl).

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Concentration Matters

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  1. 10 ng of sample (note: we request 50 ng of DNA in 10ul and run 5ul on the 3130xl) Concentration Matters Note the areas of low resolution 20 ng of sample (note: we request 50 ng of DNA in 10ul and run 5ul on the 3130xl) The call reliability has drastically increased at 20 ng in the same region

  2. Note the increased signal intensity and trace scores for 20 ng The Contiguous Read Length is the longest uninterrupted stretch of bases with quality higher than a specific limit. QV20+ value is the total number of bases in the entire trace that have basecaller quality value greater than or equal to 20 (99% probability the call is correct).

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