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Formación de EtOH a partir de Glicerol

Formación de EtOH a partir de Glicerol. Escenarios para el Biodiesel. 17000 Barriles = 969000 L de Biodiesel = 96900 L de glicerol. Problemâtica del Glicerol.

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Formación de EtOH a partir de Glicerol

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  1. Formación de EtOH a partir de Glicerol

  2. Escenariospara el Biodiesel 17000 Barriles = 969000 L de Biodiesel = 96900 L de glicerol

  3. Problemâtica del Glicerol • El glicerolcomosubproducto de la industriaimplicaunagranfuente de contaminaciôn y alto costo de procesoparadesecho del mismo sin obteneralgunarentabilidadporsuprocesado. • La granoferta y la bajademandahacen del glicerolunamateria prima de muybajoprecio.

  4. Ruta Metabólica Única Fuente de C Anaerobiosis EtOH

  5. Paso limitante para la utilización de glicerol

  6. Glicerol Quinasa • 4 Subunidades • Km= 10mM • pH = 9.5 ----- Km = 50 mM • 0°C --------Km = 25 mM • Kd= 10 mM • 3.7 mol glicerol/ mol enzima

  7. Glicerol Quinasa- Inhibición • Fructosa 1,6 bifosfato • Ki= 0.5 mM • No competitivo • Glucosa • ATP • Mg 2+ • Glicerol 3P • Ki= 0.5 mM Inhibidores Leves

  8. Glicerol 3P

  9. Estructura Primaria • 502 aa • Masa Molar: 56106 • Ac Glutámico • -Carboxil terminal • Treonina • -Amino terminal

  10. Condiciones Óptimas

  11. Enzima Mutante

  12. Microorganismos Posibles • Escherichia coli • Zymomonasmobilis • Bacillussubtilis • Saccharomycescerevisiae

  13. Bibliografía • Catalytic and Allosteric Properties of Glycerol Kinase from Escherichia coli*JEREMY W. THORNER AND HENRY PAULU Department of Biological Chemistry, Harvard Medical School, THE JOURNAL OF BIOLOGICAL CHEMISTRY. • CRECIMIENTO Y FORMACIÓN DE PRODUCTOS EN CULTIVOS AERÓBICOS Y ANAERÓBICOS DE Bacillussubtilis,CLAUDIA IBETH HERNÁNDEZ BUSTOS IBT • Tecnología de la fermentación del etanol - mobilis de Zymomonas P. Gunasekaran y K. ChandraRajDepartamento de la tecnología microbiana, escuela de ciencias biológicas, universidad de MaduraiKamaraj, Madurai • Theglycerolkinase )GUT1= gene of Saccharomycescerevisiae; cloning and characterization. P Pavlik, M Simon, T Shchuster, H. Ruis • http://www.eimaep.org/pdfs/rutas/24.pdf • Escherichia coli GlycerolKinase CLONING AND SEQUENCING OF THE glpK GENE AND THE PRIMARY STRUCTURE OF THE ENZYMEDonald W. PettigrewS, Din-Pow Ma#, Charles A. Conrad#, and James R. JohnsonnDepartment of Biochemistry and Biophysics and the $Department of Biology, Texas A&M University

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