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Laser-assisted Thermal-expansion Microinjector

Laser-assisted Thermal-expansion Microinjector. LTM-1000. Conventional Microinjector. Inject by oil pressure or gas pressure If the capillary with less than 0.5 μm tip is used, the capillary itself will be moved forward due to the pressure. With complicated flow path.

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Laser-assisted Thermal-expansion Microinjector

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  1. Laser-assisted Thermal-expansion Microinjector LTM-1000

  2. Conventional Microinjector • Inject by oil pressure or gas pressure • If the capillary with less than 0.5 μm tip is used, the capillary itself will be moved forward due to the pressure. • With complicated flow path • Unable to use fine tips under 0.5 μm for injection • Unable to inject into the cell having rigid cell wall such as plant cells • Complicated flow path brings on a time-consuming preparation

  3. LTM-1000 : Structure LTM-1000 Heart-induced expansion of liquid in a sealed capillary generates high pressure. Feature • Extremely-high pressure (approx. 1MPa per 1℃) makes it possible to use capillaries with the tip around 0.1-0.5μm without clogging. • Simple flow path allows to start experiments right away saving a lot of labor.

  4. Applications Alexa 488 Conventional Method LTM-1000 Alexa 546-dextran 10K Alexa 546-dextran 10K 50µm 20µm Injection of antibody into plant cells 50µm Lucifer Yellow Injection into the female gametophyte of plant *Whereas the damage and sample escape appear by the conventional method, LTM-1000 ensures no damage and continuous injections without sample escape by using a fine tip capillary. Chloroplast 5µm Injection into chloroplasts of Cyanidioschyzon merolae

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