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Whole Blood Centrifuged with Anticoagulant

Whole Blood Centrifuged with Anticoagulant. Plasma. (54%). Serum. - plasma minus fibrinogen. H 2 O. -91-92%. Protein. -7-8%. Electrolytes. Nutrients. Blood Gases. 6. 3. Hormones. Red Cells. - 5 X 10. / mm. 3. 3. White Cells. - 6-9 X 10. / mm. Buffy Coat. (1%).

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Whole Blood Centrifuged with Anticoagulant

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  1. Whole Blood Centrifuged with Anticoagulant Plasma (54%) Serum - plasma minus fibrinogen H2O -91-92% Protein -7-8% Electrolytes Nutrients Blood Gases 6 3 Hormones Red Cells - 5 X 10 / mm 3 3 White Cells - 6-9 X 10 / mm Buffy Coat (1%) (white cells and platelets) Hematocrit (volume RBC’s/unit plasma) Red Cells (45%) (erythrocytes) males - 40-50% females- 35-40%

  2. Red Blood Cells (erythrocytes) Biconcave Discs 7.5-8.5 um dia. rapidly-flowing blood stagnant blood- rouleaux Sickle Cells (valine-glutamine subst. b-hemoglobin chain) Erythrocyte Shape Varies with Tonicity Spherocytosis (spectrin alteration) hypertonic isotonic hypotonic very hypotonic (spheres) (ghosts)

  3. Microscopy of Erythrocytes Blood Smear- erythrocytes SEM- erythrocytes

  4. BLOOD PLATELETS (thrombocytes) micro- tubules microtubules granules glycogen Hyalomere Granulomere (pale blue) (purple granules) Platelet- light micrograph glycogen microtubules microtubules

  5. Blood Clotting Prothrombin Thromboplastin (released from platelets and injured vessel wall) Thrombin Fibrinogen Fibrin (clot) (feltwork of fibrils, platelets thrombus and blood cells) lesion platelets

  6. AGRANULAR LEUKOCYTES Lymphocytes (20-25%) Monocytes (3-8%) B Lymphocytes (humoral immunity) Mononuclear T-Lymphocytes Phagocytic System (cell mediated immunity) Monocytes Lymphocytes

  7. GRANULAR LEUKOCYTES (55-65%) Neutrophil (PMN) (polymorphonuclear leukocyte) Specific Granules Lobes (lysosomes & phagocytins0 of Azurophil Granules Nucleus (lysosomes) Ectoplasmic Layer Neutrophil Pseudopodia Specific Granules

  8. Erythrocytes, Lymphocytes, Neutrophils and Platelets Bar Body

  9. GRANULAR LEUKOCYTES Eosinophils (1-3%) Eosinophilic (acidophilic) granules (destroy antigen-antibody complexes) Crystals Eosinophil Specific Granules

  10. Granular Leukocytes Basophils (less than 1%) Basophilic Granules heparin histamine serotonin Basophil

  11. Eosinophil, Neutrophil and Basophil Eosinophil Neutrophil Basophil

  12. Blood Plasma Plasma- transparent, yellow liquid vehicle for cells Serum- plasma without fibrinogen Serum Proteins: Albumin (@ 60 K) - colloid osmotic pressure and provide for solubility of hydrophobic components (e.i. lipids) Globulins (> 80 K) gamma globulins- e.i. IgG (immune globulins-antibodies) beta globulins - hormone transport (e.i. thyrogloblin) Transferrin- iron transport Cerruloplasmin- copper transport Serum Lipoproteins chylomicrons- fatty acid transport Very Low Density Lipoproteins (VLDL)) Low Density Lipoproteins (LDL) High Density Lipoproteins (HDL)

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