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-omab

Mouse spleen cells. Hybridoma. MOUSE. Ag Immunization. 1986. (Muromonab). Screening mouse Ig. Cell colture. -omab. Myeloma cells. hCH2. hCH1. hCL. mVL. hCH3. mVH. Mouse VH and VL. +. CHIMERIC. 1994. (Abiciximab). -ximab. Mouse mAb. Human CH and CL. 1994.

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-omab

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  1. Mouse spleen cells Hybridoma MOUSE Ag Immunization 1986 (Muromonab) Screening mouse Ig Cell colture -omab Myeloma cells hCH2 hCH1 hCL mVL hCH3 mVH Mouse VH and VL + CHIMERIC 1994 (Abiciximab) -ximab Mouse mAb Human CH and CL 1994 Mouse CDR grafting (Daclizumab) Human mAb mCDR3 mCDR2 mCDR3 mCDR1 mCDR2 mCDR1 HUMANIZED + Human backbones 1998 (Palivizumab) -zumab Vh+Vl genes amplification Construction of antibody library and display on phage surfaces 1) Decreasedimmunogenicity 2002 (Adalimumab) From phage display Graft human IgG genes into mice Produce human mAbs by hybridoma technology HUMAN 2) Human source 2006 Screening Human IgG (Panitumumab) From transgenic mouse Isolate B cells from patients ? From EBV immortalization Immortalization 3) + -umab = EBV CpG

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