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MLAB 2434 – CLINICAL MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & KERI BROPHY

MLAB 2434 – CLINICAL MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & KERI BROPHY. Chapter 8 – Microscopic Examination of Infected Materials. Chapter – 8 - Microscopic Examination of Infected Materials (cont’d). Purposes for direct gram stain from patient specimens

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MLAB 2434 – CLINICAL MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & KERI BROPHY

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  1. MLAB 2434 – CLINICAL MICROBIOLOGYSUMMER, 2005CECILE SANDERS & KERI BROPHY Chapter 8 – Microscopic Examination of Infected Materials

  2. Chapter – 8 - Microscopic Examination of Infected Materials (cont’d) • Purposes for direct gram stain from patient specimens • Confirm that specimen submitted is representative (ex. Sputum vs. spit) • Determine probability of infection by identifying debris of inflammation • Identify specific infectious agents

  3. Chapter – 8 - Microscopic Examination of Infected Materials (cont’d) • Guide initial antibiotic therapy • Use for epidemiologic data • Direct gram stains observed with light microscope after staining • Preparing smears • Using swabs • Smears should not be made using swab that has been used to streak cultures

  4. Chapter – 8 - Microscopic Examination of Infected Materials (cont’d) • Ideally, if culture AND gram stain are to be done, two swabs of the specimen should be collected and sent to the lab • Swab is NOT rubbed on the slide, but rolled back and forth (p. 263) • Smears from thick liquids or semisolids (ex: stool) • Smears from thick or mucoid material • “Pull” slides

  5. Chapter – 8 - Microscopic Examination of Infected Materials (cont’d) • Smears from thin fluid (ex: CSF) • Mark back of slide with marking pen to show location of specimen on the slide for ease in finding under microscope • Cytocentrifuge

  6. Chapter – 8 - Microscopic Examination of Infected Materials (cont’d) • Examination of Prepared and Stained Slides • If sputum, first evaluate the smear to determine if it is REALLY sputum • Unacceptable = 10-20 squamous epithelial cells/LPF • Acceptable = 10-25 PMN/LPF • Evaluate smear first under low power to look for background material, such as WBC (pus), epithelial cells, etc. • Search for microorganims

  7. Chapter – 8 - Microscopic Examination of Infected Materials (cont’d) • http://www.medtraining.org • For your ID, use your email address • Your password is your first name and first letter of your last name, all lower case

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