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ABSTRACT

ABSTRACT. Determination of paracetamol and Codeine Phosphate simultaneously using the reversed phase High Performasce Liquid Chromatography (HPLC) have been performed. The mobile phase solution obtained can separates paracetamol from

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ABSTRACT

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  1. ABSTRACT Determination of paracetamol and Codeine Phosphate simultaneously using the reversed phase High Performasce Liquid Chromatography (HPLC) have been performed. The mobile phase solution obtained can separates paracetamol from codeine phosphate with good resolution (RS = 2,704), this mobile phase were a mixture of methanol and phosphate buffer 20 mM pH 2,5 (15:85). The condition of HPLC used were Zorbax C8 column, temperature 27oC, flow rate 1,5 ml/minutes and detected by DAD (λ = 284 nm). Linearity of paracetamol and codeine phosphate were good (r paracetamol 0,9999 with Vxo = 0,51 % and r codeine phosphate 0,9999 with Vxo 1,22 %). Precision instrument for paracetamol and codeine phosphate were 0,77% and 0,79 % respectively. Accuracy of the methods were in the range of accepted limits ( 98 - 102 % ), average recovery of paracetamol and codeine phosphate in the simulation matrix were 99,99 % and 100,5 % respectively. Precision methods of paracetamol 0,64% and codeine phosphate 0,75%. The concetration of paracetamol and codeine phosphate in sample tablet were 100,8% and 99,52% respectively, that fulfill of USP XXIV requairement ( 90 - 110 % ). Keyword : HPLC, Paracetamol, Codeine phosphate, Optimation of mobile phase.

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