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Supplementary data 1: Detailed characteristics of OsSce1 and OsSce2 proteins.
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Supplementary data 1: Detailed characteristics of OsSce1 and OsSce2 proteins.
Supplementary data 2: Detailed characteristics of rice FKBP proteins. The protein sequences were retrieved from the TIGR database (www.tigr.org). The molecular weight and isoelectric point were determined using EditSeq module of DNAstar. Localization was predicted based on transit peptide sequence using TargetP 1.1 (http://www.cbs.dtu.dk/services/TargetP/) and PSORT (http://psort.nibb.ac.jp/) databases. NLS prediction was done at PredictNLS server (http://cubic.bioc.columbia.edu/predictNLS/). The protein sequences retrieved from the TIGR database were used for domain analysis at the Pfam database (http://www.sanger.ac.uk/Software/Pfam/search.shtml). The names proposed in the brackets are the calculated molecular weight of the proteins based on their cDNAs. Os01g38180 (OsFKBP57-1) 57.3 5.42 Cytoplasm Locus ID Molecular weight (kDa) Isoelectric point Localization Domain architecture Os01g38229 (OsFKBP47) 47.3 4.60 Nucleus Os01g38359 (OsFKBP45) 45.6 5.17 Cytoplasm Os01g62610 (OsFKBP20.1) 20.1 5.98 Nucleus 16.8 9.59 Mitochondria Os01g68710 (OsFKBP16) 22.2 6.88 Cytoplasm Os02g07220 (OsFKBP22) Os02g28980 (OsFKBP74) 74.8 5.11 Cytoplasm Os02g51570 (OsFKBP23) 23.4 8.91 Chloroplast 12.08 7.96 Nucleus Os02g52290 (OsFKBP12) Os03g25140 (OsFKBP70) 70.4 5.37 Cytoplasm 65.56 5.31 Cytoplasm Os04g28420 (OsFKBP65)
Locus ID Molecular weight (kDa) Isoelectric point Localization Domain architecture 57.17 5.88 Nucleus Os04g36890 (OsFKBP57-2) Os07g09040 (OsFKBP60-1) 60.0 8.02 Chloroplast 64.1 5.06 Cytoplasm Os08g41390 (OsFKBP64) 19.8 7.19 Nucleus Os05g38370 (OsFKBP20) Os08g42850 (OsFKBP27) 27.1 8.23 Chloroplast 21.9 9.27 Chloroplast Os06g45340 (OsFKBP21) 60.1 4.93 Cytoplasm Os09g01650 (OsFKBP60-2) Os09g01670 (OsFKBP44-1) 44.3 5.18 Mitochondria Os09g12270 (OsFKBP44-2) 44.1 5.82 Nucleus Os09g32526 (OsFKBP16) 16.2 5.12 Extracellular 42.4 6.16 Cytoplasm Os11g05090 (OsFKBP42-1) 42.2 5.67 Cytoplasm Os12g05090 (OsFKBP42-2) Signal peptide NLS TPR FKBP
Supplementary data 3: A protein phylogenetic tree was created according to the neighbor-joining method clustering strategy in ClustalX program and analyzed using TREEVIEW 1.6.5 software. The proteins falling under the same group are shown in same color. Os08g42850 At2g43560 Os09g12270 At5g45680 At2g12340 Os04g36890 Os06g45340 Os04g36890 At4g39710 At5g05420 Os02g51570 Os09g01670 At3g10060 Os07g09040 Os09g01650 At5g13410 At4g25340 At3g60370 At1g18170 At1g73655 At4g19830 At5g55220 Os02g07220 Os01g38359 At1g20810 Os01g38180 Os12g05090 Os01g38229 Os11g05090 Os02g28980 At3g21640 At5g48570 At3g25230 Os01g62610 Os04g28420 At3g54010 Os05g38370 Os08g41390 Os03g25140 At5g64350 Os09g32526 At 3g55520 Os01g68710 At 5g48580 At3g25220 Os02g52290
Consensus Consensus Consensus Consensus Consensus OsFKBP20 OsFKBP20 OsFKBP20 OsFKBP20 OsFKBP20 NP_014264 NP_014264 NP_014264 NP_014264 NP_014264 NP_191111 NP_191111 NP_191111 NP_191111 NP_191111 AAF18387 AAF18387 AAF18387 AAF18387 AAF18387 NP_1075779 NP_1075779 NP_1075779 NP_1075779 NP_1075779 AAH03447 AAH03447 AAH03447 AAH03447 AAH03447 AAH68678 AAH68678 AAH68678 AAH68678 AAH68678 Supplementary data 4: Alignment report of the OsFKBP20 protein with other eukaryotic FKBPs.NP_014264- Saccharomyces cerevisiae; NP_191111- Arabidopsis thaliana; AAF18387- Drosophila melanogaster; NP_1075779- Oryctolagus cuniculus; AAH03447- Mus musculus; AAH68678- Xenopus laevis.
Consensus Consensus Consensus OsFKBP20 OsFKBP20 OsFKBP20 NP_014264 NP_014264 NP_014264 NP_191111 NP_191111 NP_191111 AAF18387 AAF18387 AAF18387 NP_1075779 NP_1075779 NP_1075779 AAH03447 AAH03447 AAH03447 AAH68678 AAH68678 AAH68678