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The Story of Spontaneous Generation

The Story of Spontaneous Generation. Spontaneous generation. Spontaneous generation is the idea that living things can be produced from non-living things.

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The Story of Spontaneous Generation

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  1. The Story of Spontaneous Generation

  2. Spontaneous generation • Spontaneous generation is the idea that living things can be produced from non-living things. • From the time of the ancient Romans, through the Middle Ages, and until the late nineteenth century, it was generally accepted that some life forms arose spontaneously from non-living matter.

  3. The Question • Where did life forms such as mice, maggots, and microorganisms come from?

  4. Different Theories of Spontaneous Generation • Theory 1 = the spontaneous production of mice required placing sweaty underwear and husks of wheat in an open-mouthed jar, then waiting for about 21 days, during which time it was alleged that the sweat from the underwear would penetrate the husks of wheat, changing them into mice.

  5. The Theories • T2 = maggots arise spontaneously in rotting meat • T3 = microorganisms arise spontaneously in broth.

  6. Different scientist tested theses theories over the years • Francesco Redi • An Italian doctor • Louis Pasteur • A French Scientist • Lazzaro Spallanzani • An Italian Scientist

  7. Redi’s Experiment • Question: where did maggots come from • Hypothesis: maggots came from the eggs of flies

  8. Redi’s Experiment • Procedure: Redi put rotting meat into three jars. • Jar 1 – open to the air • Jar 2 – covered with netting • Jar 3 – sealed off completely from outside

  9. Redi’s Experiment • Results: • Jar 1 = maggots developed on rotting meat • Flies were observed on meat

  10. Redi’s Experiment • Results • Jar 2 = maggots appeared on netting • Flies were observed on netting

  11. Redi’s Experiment • Results • Jar 3 = maggots did not appear at all in or around the sealed jar • No flies were observed near jar 3

  12. Redi’s Experiment • Conclusion: • The hypothesis was supported by the data and therefore accepted • Because there were no maggots in or around the jar it was concluded the maggots came from the fly eggs.

  13. Spallanzani’s Experiment • Question: What causes microbes to form from decaying broth? • Hypothesis: Microbes come from the air. Boiling will kill microbes.

  14. Spallanzani’s Experiment • Procedure: • Spallanzani put broth into four flasks • Flask 1 – left open to the air • Flask 2 – was sealed • Flask 3 – was boiled and left open • Flask 4 – was boiled then sealed

  15. Spallanzani’s Experiment • Results • Flask 1 – left open to the air • Turned cloudy (microbes found) • Flask 2 – was sealed • Turned cloudy (microbes found) • Flask 3 – was boiled and left open • Turned cloudy (microbes found) • Flask 4 – was boiled then sealed • Did not turn cloudy (NO MICROBES)

  16. Spallanzani’s Experiment • Conclusions: • The hypothesis was accepted • Because the combination of boiling the broth (killing bacteria that were in the jar to start) and sealing the jar (keeping bacteria in the air out) it was concluded that boiling does kill bacteria and that Microbes come from the air.

  17. Pasteur’s Experiment • Question: Where do microbes come from to cause chicken broth to decay? • Hypothesis: Microbes come from cells of organisms on dust particles in the air; not from the air itself.

  18. Pasteur’s Experiment • Procedures: • Control Group • One flask of broth open to the air • Experimental group • One S-shaped flask that prevents dust from entering the flask.

  19. Pasteur’s Experiment • Procedure: • Pasteur put broth in both the open flask and into special S-Shaped flasks and boiled it. • S-shaped flask was used to trap any dust particles • Boiling was done to kill all the microbes already in the flask.

  20. Pasteur’s Experiment • Procedure (cont.) • Pasteur then watched to see if any changes took place with the appearance of the broth in the flasks. • If the broth became cloudy that meant microbes were present

  21. Pasteur’s Experiment • Results: • Open flask – the broth became cloudy • Closed flask – the broth remain clear • Dust was observed in the neck of the S-shaped neck of the flask.

  22. Pasteur’s Experiment • Conclusion: • The hypothesis was supported by the data therefore it was accepted. • Because the broth did not turn cloudy and dust was observed trapped in the neck of the S-Shaped flask, it was concluded that the microbes came from cells of organisms riding on dust particles.

  23. The Scientific Method • Each of these scientist used the scientific method of investigation to successfully disprove spontaneous generation. • Remember Science is not just a body of facts but a method of investigation.

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