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Dendritic cells and mast cells : their role in the progression of the Mycosis Fungoides

Dendritic cells and mast cells : their role in the progression of the Mycosis Fungoides. Salvatore Marasà ¹, Daniela Cabibi ¹, Lorenzo Marasà ², Federico Aragona ¹. 1 Institute of Pathologic Anatomy, University of Palermo, Italy.

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Dendritic cells and mast cells : their role in the progression of the Mycosis Fungoides

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  1. Dendritic cells and mast cells : their role in the progression of the Mycosis Fungoides Salvatore Marasà ¹,Daniela Cabibi¹, Lorenzo Marasà ²,Federico Aragona¹. 1 Institute of Pathologic Anatomy, University of Palermo, Italy. 2 C.O.U. of Pathologic Anatomy, ARNAS-Civico Hospital, Palermo, Italy.

  2. Mycosis fungoides (MF) is a special type of cancer, in which the neoplastic cells and those responsive to them are represented by the same cell: lymphocytes. The prolonged course of disease and the bystander intense infiltrate suggest a strong antineoplastic immune response.

  3. Progression of the Mycosis Fungoides PATCH STAGE PLAQUE STAGE TUMOUR STAGE

  4. Aims We studied the role of the epidermal Langherans cells(LCs), dermal dendritic cells (DDCs) and the mast cells (MCs) in the progression of the MF.

  5. Materials and methods The study included 32 patients. The immunoreactivity was investigated quantitatively and topographically using monoclonal antibodies CD3,CD4,CD8,CD1a,Lag,Langerina,S100,Tryptase, cKit,CD31,CD34,FactorVIII,VEGF. The average number of positive cells was calculated for 5 fields with higher cell density at 400x. The intensity of cell immunostaining was scored as 0(0-5 cells),1 (5-10 cells) and 2 (+10cells). The neovascularisation was studied using MAGS and Microvessel Density.

  6. Results LCs were significantly increased in plaque stage; conversely during tumour stage they decreased to the point that microascessi were almost free. To compensate for this reduction, however, during the nodular period, we noted an increase in the population of DDCs. MCs and neoangiogenesis was accentuated in tandem with the progression of MF.

  7. Epidermal Langherans Cells (LCs), Dermal Dendritic Cells (DDCs )CD1a+ Patch Stage Tumour stage

  8. Neovascularisation Neovascularisation and Mast Cells (MCs) Patch stage Tumour stage Plaque stage E.E. Tryptase

  9. Conclusions This study support the dual role of LCs and DDCs in the MF: in the early stages they are involved in the host defense against malignant T lymphocytes, while in advanced stages they are involved in a immune fall of the host to which they themselves would be involved. Besides the neovascularization closely is correlated with tumour progres-sion and amount of perivascular tryptase-positive MCs .

  10. References • 1.Girardi M. et al. : N Engl J Med 2004,350:1978-1988. • 2.Kim E.J. et al.: J Clin Invest 2005,115:798-812. • 3.Marasà L.et al.: Pathologica1983,75:353-365. • 4.Weidner N. et al.: N Engl J Med 1991,324 : 1-8.

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