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Biological Stains. Chromophore – colored molecules Auxochrome – modifies chromophore to make it reactive (bind). Negative charge = acidic = anionic Positive charge = basic = cationic. Bacterial membranes and most bacterial components have a slight negative charge
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Biological Stains Chromophore – colored molecules Auxochrome – modifies chromophore to make it reactive (bind)
Negative charge = acidic = anionic • Positive charge = basic = cationic
Bacterial membranes and most bacterial components have a slight negative charge • Therefore, most useful stains are basic (positive) • Acidic stains, such as eosin, are used for particular purposes, and rarely used to stain bacteria
