Jae Young Lee, Christine E. Schmidt

1. Electrochemically coated hyaluronic acid on conducting substrates through pyrrole moieties for the potenital use of neural proves. Jae Young Lee, Christine E. Schmidt

2. Figure 1. Chemistry

3. Figure 2. Electrochemical synthesis ITO glass slide 1. PDMS well on ITO PDMS well (1st) 2. Electrochemical deposition of pyrrole-HA 3. Placing culture with a larger PDMS well Bare ITO glass PPy-HA deposited area PDMS well (2nd) 4. Cell culture

4. Figure 3. Electrochemical synthesis a b Electrochemical deposition on ITO slides in 1% (w/v) aqueous solutions of HA (a) and pyrrole-HA (b). 20 scans was performed at a scan rate of 0.05 V/min from 0 to 1.0 V vs SCE.

5. Figure 4. Water contact angle a b Water contact angles were measured on a uncoated-ITO glass slide (a) and pyrrole-HA deposited slides (b). After electrochemical polymerization, ITO surface became hydrophilic, showing lower water contact angle (38.1±4.4°) compared to that of uncoated bare ITO (85.5±1.1°) . c

6. Figure 5. biotinylated HABP binding protein / Cy3-streptavidin a b Fluorescence images of substrates treated with bHAPA (biotinylated hyaluronic acid binding protein) and Cy3-streptavidin: bare ITO slide (a), pyrrple-HA deposited ITO without (b) and with (c) bHABP treatment, and boarder between bare ITO and pyrrole-HA coated (d). c d ITO pyrrole-HA

7. Figure 6. XPS a b c d High-resolution spectra of carbon (a) and nitrogen (b) atoms of pyrrole-HA deposited ITP slides. Typical HA peaks were observed in C1s spectrum: (C-C/C-H, at 284.67 eV (FWHM=1.196 eV, 3% GL; C-N/C-O at 286.30 eV (FWHM=1.432 eV, 38% GL); O=C-O at 288.34 eV (FWHM=1.523, 0% GL). Nitrogen peak was newly detected at 399.8 eV.

8. Figure 7. AFM a b c Thickness ~5 nm

9. Figure 8. Astrocyte culture a b c d ITO HA-coated

10. Figure 9. Astrocyte culture long term culture