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Characterization of the ASPL-TFE3 translocation and Junction -Specific siRNA Targeting in Alveolar Soft Part Sarcoma (ASPS). ASPL. TFE3. 21nt. Balanced Translocation. Unbalanced Translocation. Translocation-Specific siRNA design. Xenograft. Neo. P CMV. LUC. (FU-UR-1)
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Characterization of the ASPL-TFE3 translocationand Junction-Specific siRNA Targeting in Alveolar Soft Part Sarcoma (ASPS) ASPL TFE3 21nt Balanced Translocation Unbalanced Translocation Translocation-Specific siRNAdesign Xenograft Neo P CMV LUC (FU-UR-1) 3T3 Fibroblasts Hyg P CMV TFE3 Puro P CMV Soft Agar Assay ASPL-TFE3 siRNAtransfection siRNA injection qPCR Tumor Size Vector map: Retro X System and Vectors (Clonetech, Mountain View, CA , USA) Mouse Image: Maestro in vivo imaging system.(CRI, Woburn, MA, USA) Chromosomes: Graphic template used to create translocation figure borrowed from US National Library of Medicine Nenad Svrzikapa