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Сombination of Nanoparticles and Photosensitizers in Photodynamic Action on Microorganisms

S aratov S tate U niversity. Сombination of Nanoparticles and Photosensitizers in Photodynamic Action on Microorganisms Pavel O. Petrov, Ivan A. Orlov, Alexandr V. Kovalev, Elena S. Tuchina and Valery V. Tuchin. Laboratory of Bacteriology Res.-Edu. Institute of Optics and Biophotonics

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Сombination of Nanoparticles and Photosensitizers in Photodynamic Action on Microorganisms

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  1. Saratov State University • Сombinationof Nanoparticlesand Photosensitizers • in Photodynamic Action on Microorganisms • Pavel O. Petrov, Ivan A. Orlov, Alexandr V. Kovalev, • Elena S. Tuchina and Valery V. Tuchin Laboratory of Bacteriology Res.-Edu. Institute of Optics and Biophotonics Saratov State University

  2. Goal: To study the sensitivity of staphylococci to the combined effects of light, dyes and nanoparticles

  3. Genera Staphylococcus: Staphylococcus aureus Staphylococcus epidermidis • Gram-positive cocci arranged in clusters • Occurs frequently on the skin and mucous membranes of humans and animals • Cause of acne, boils, endocarditis, osteomyelitis, toxic shock syndrome, nosocomial infections, wound infections, etc. • Often resistant to a wide variety of antibiotics, including methicillin • Contains carotenoids, staphyloxanthin, 4,4'-diaponeurosporene

  4. Bacteria strains • S. aureus 209 P • S. epidermidis • two strains – meticillin-susceptible (MSSE) and meticillin-resistance (MRSE) • Staphylococci were grown on nutrient agar and incubated at 37°C

  5. Experimental combinations Blue and Red LEDs + Methylene Blue + Titanium Dioxide Blue and Red LEDs+ Brilliant Green + Titanium Dioxide Infrared laser + Indocyanine Green + Titanium Dioxide For the treatment of infections on the surface of skin and mucous For the treatment of infections in the tissues

  6. Characteristics and parameters Light Exposure:5, 10, 15, 30 min

  7. Bacterial suspension (10 000 mc/ml) (Fig. 1) Bacterial suspension in PBS (1000 mc/ml) Bacterial suspension in dye water solution (1000 mc/ml) Fig. 1 Darkness (control) Darkness (control) Light exposure Light exposure (Fig. 2) Fig. 2 24-48 h incubation 37 C on dense nutrient medium º Counting the number of colony forming units, using the original digital program (Fig. 3 ) Fig. 3

  8. The action of Blue+Red LEDs, Methylene Blue and TiO2 on microbial colony forming ability

  9. The action of Blue+Red LEDs, Brilliant Geen and TiO2 on microbial colony forming ability

  10. The action of IR laser, Indocyanine Green and TiO2 on microbial colony forming ability

  11. Summary • The most effective combination for inhibition the growth of the three investigated staphilococci is the LED light (405, 625 nm), Brilliant Green and TiO2 nanoparticles. • This bacteria killing technology has several advantages: • a low power radiation and low concentration of dyes can be used; • the effectiveness is achieved through various mechanisms (photodynamic, photocatalytic, photothermal) that prevents the formation of bacterial resistance

  12. Acknowledgments • The research described in this paper has been made possible by: • Grant 224014 PHOTONICS4LIFE of FP7-ICT-2007-2 • Grant of President of RF "Supporting of Leading Scientific Schools" - NSHA-208.2008.2 • Projects 2.1.1/4989 and 2.2.1.1/2950 of RF Program on the Development of High School Potential • Project 1.4.09 of Federal Agency of Education of RF • The Special Program of RF "Scientific and Pedagogical Personnel of Innovative Russia," Governmental contracts: 02.740.11.0484 and 02.740.11.0770 • We are grateful to: • Docent Dolotov L.E. (SSU) for the development of the LED light device

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