1 / 1

HCl 1M 1M 1M 2M 2M 2M 2M

A. B. K. Q. R. HCl 1M 1M 1M 2M 2M 2M 2M temp 20 60 70 70 80 80 100° C min 30 30 30 30 30 60 60. 0M 1M 2M 2M 20 70 80 100° C

keagan
Download Presentation

HCl 1M 1M 1M 2M 2M 2M 2M

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. A B K Q R HCl 1M 1M 1M 2M 2M 2M 2M temp 20 60 70 70 80 80 100° C min 30 30 30 30 30 60 60 0M 1M 2M 2M 20 70 80 100° C 30 60 60 60 C 4mm HCl (1M) + - + + temp 20 70 70 100° C DPBA - + + + Figure S1. Test of conditions for deglycosylaton of rutin and Brassica anther flavonoids. Panels A and B show TLC separations of flavonoids after rutin (quercetin-3-rhamnose-glucose) or a stage-5 Brassica anther extract, respectively, was deglycosylated in 0-2 M HCl at different temperatures for 30 or 60 min. The TLC plates after DPBA staining were placed on top of a UV irradiation source and photographed. Q, K and R denote quercetin, kaempferol and rutin, respectively. Released quercertin and kaempferol fluoresced, whereas unreacted rutin and glycosylated flavonoids at the origin of the TLC plates did not. Panel C shows CLSM images of stage-5 Brassica tapetum cells after different conditions of acid deglycosylation and DPBA staining. Fluorescence is shown in green, and the cell circumference is marked with a dotted white line. All images in Panel C were taken with identical CLSM settings (laser power and detection gain). The fluorescent particles in the 100 0C-treated cell had surface extensions (arrowed) that were absent in those in the 70 0C-treated cell.

More Related