Ron Woodruff

1. Jim Thompson Ron Woodruff Clay Hallman

3. These creatures are hard to photograph! (Jim holds the camera, and Clay is adept at avoiding it.) Jim is a David Ross Boyd Professor of Biology at OU, and Clay is a Research Associate. Jim and Ron have taught this course for about 24 years, and this is Clay’s 10th year as the Teaching Assistant.

4. Ron is a Distinguished Research Professor at Bowling Green State University in Ohio. (Ron is simply able to focus on other things and, thus, ignore the camera.)

5. The University of Oklahoma Biological Station is located on the quiet shore of Lake Texoma, at one time the largest man-made reservoir in the world.

6. But then, at other times, it was not quite so quiet!

7. We worked in two adjacent rooms on the first floor of the classroom building. The Station has wonderful resources for small, intensive classes like ours, as well as support for year-round graduate student and faculty researchers.

8. Our activities were split between a lecture/writing room and a separate lab, where chemicals and other potential hazards could be safety used and monitored.

9. They also have good food! That is good, because there is not much else around for dozens of miles.

10. Molecular Techniques for Field Biology Brad Snider Danielle Vinnedge Rachel Flanagan Robert Byrd Danielle Strawn Jennie Lee

11. Molecular Techniques for Field Biology Taylor Paziuk Adam Richardson Barun Bouvia Zain Hyder James Pembrook John Logan

12. Although we had four “Lab Groups”,in practice everyone worked together. Green: Red: Robert Byrd Brad Snider Danielle Vinnedge Rachel Flanagan John Logan James Pembrook Brown: Blue: Barun BouviaZain Hyder Danielle Strawn Jennie Lee Taylor Paziuk Adam Richardson FRONT

13. Barun Bouvia Zain Hyder Danielle Strawn

15. Robert Byrd Brad Snider Danielle Vinnedge

17. Rachel Flanagan John Logan James Pembrook

19. Jennie Lee Taylor Paziuk Adam Richardson

21. Who would have thought spots could be so much fun?

23. Seining will probably not become a popular spectator sport.

24. Is it still called “field” work when it is wet?

25. Such a big lake … such little nets. Eventually, we caught one minnow. (We believe in quality, not quantity – although quantity would not have been turned away)!

26. The whole class prepared successful protein electrophoresis plates from different parts of this now-famous minnow.

27. It’s all in the wrists!

28. A nice combination of individual and team work.

29. Back safely from another field trip – (to the lab down the road)

30. At the Station we are surrounded by biology.

31. Our data were put into the appropriate theoretical framework. (Our jokes, however, were in a class of their own.)

32. Preparing for a guest lecture by Dr. Philip Morton. He engaged us with a research project that involved us in generating hypotheses and applying our new knowledge.

33. Using molecular genetic databases … there is a world of data out there!

34. Results from our BarCoding of tissue samples by PCR Sample 5 >MT1-5_HCO TATCTTGGTATAAATAGGGTCTCCTCCTCCAGCAGGATCAAAGAATGATGTATTAATATTTCGGTCTGTTAAGAGTATTGTGATTGCTCCGGCGAGTACGGGTAGAGAAA GAAGTAATAATAAGGCAGTTAAAGCTACTGATCAGACAAATAGAGGCATTCGATCAAAAGTTATTCCTGTAGAACGCATATTAATAACAGTTGTAATAAAATTTACAGCCC CGAGGATTGATGAGATTCCTGCAAGGTGAAGCCTAAAAATAGCTAAATCTACAGAAGCCCCTCTATGAGCGATATTAGAGGATAATGGCGGGTAAACAGTTCAACCTGT CCCGGCCCCTCTTTCTACAAGGCTTCTCATTAAAAGAAGAGTTAGGGAAGGGGGTAGTAATCAAAATCTTATATTATTTATTCGAGGGAATGCTATATCAGGGGCACCCA ATATAAGTGGTACAAGCCAATTTCCAAACCCTCCAATTATAATAGGTATTACTATAAAGAAAATTATTACAAATGCATGAGCAGTTACAATAACATTATAAATTTGGTCGTCT CCGATTAATGTTCCCGGATTTCCAAGTTCAGCTCGAATAAGTAATCTTAATGAGGTTCCAACTATACCTGCTCATCTTCCAAAGAGAAAGTATAAGGTTCCAATATCTTTAT BLAST result: Phyllophaga crassissima Cytochrome Oxidase I (COI) Sequences BLAST result: Chironomus entis BLAST result: Coccinella septempunctata BLAST result: Drosophila suzukii BLAST result: Lucilia coeruleiviridis BLAST result: Leptoglossus occidentalis

35. BLAST result: Melanoplus BLAST results: Solenopsis invicta BLAST result: Larinioides cornutus BLAST result: Solenopsis invicta BLAST result: Estigmene acrea BLAST result: Phidippus audax

36. preparing a research talk on a paper using various molecular techniques – our “final exam”

37. Molecular Techniques for Field Biology – 2013