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Chapter 10. Do Now: Read 182-183. 1 ) Define bacteriophages 2) Why are viruses on the fence between life and nonlife ? 3) What are we going to explore in this chapter ? . 10.1 Experiments that showed that DNA is the genetic material. 1928: Griffith
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Do Now: Read 182-183 • 1) Define bacteriophages • 2) Why are viruses on the fence between life and nonlife? • 3) What are we going to explore in this chapter?
10.1 Experiments that showed that DNA is the genetic material • 1928: Griffith • Two strains of a bacteria (one strain caused pneumonia and the other strain was harmless) • Exp: heat killed harmful bacteria mixed with living harmless bacteria • Result: living harmful bacteria • What can we conclude from this experiment?
1940: Scientists knew that eukaryotic chromosomes were made of protein and DNA • Why did most scientists think that protein was the genetic material? • Proteins • are made up of 20 different amino acids • Can have complex structure and functions • DNA is simple • Four nucleotides
1952: Alfred Hershey& Chase pg 185 • T2 virus is made of DNA and protein • Batch 1) Grew virus in radioactive* sulfur (proteins contain sulfur and DNA does not) • Batch 2) Grew virus in radioactive* phosphorus (phosphate w/oxygen) • Steps: • 1) Allowed batches to attack bacteria • 2) Shook off any outside viral parts • 3) Spun with centrifuge, cells to the bottom • 4) Measured radioactivity in liquid vs. pellet • Result: Batch 1: protein* on the outside Batch 2: DNA* in cell • Conclusion:
10.2: DNA and RNA Description • What is DNA? A biological code to make proteins that can be passed from one generation to the next • Deoxyribonucleic Acid • DNA is made up of nucleotides (sugar (deoxyribose, phosphate and a nitrogenous base) • Polynucleotide is many nucleotides join to form a long chain of DNA • RNA uses ribose as sugar, uracil in place of thymine, and is single stranded
10.3 DNA 3D model discovered by Watson/ Crick and Rosalind Franklin What does DNA look like? A double helix- similar to a twisted ladder • The sides are made up of the sugar and phosphates (backbone) • The rungs of the ladder are made up of the nitrogenous bases held together by hydrogen bonds • The four bases are Adenine (A), Guanine (G), Thymine(T) and Cytosine (C) • Complementary base pairing Pyrimidine- purine • Adenine bonds to thymine (T-A) • Guanine bonds to Cytosine (G-C) • DNA is wrapped around proteins called histones to create the condensed form called chromatin • DNA is able to mutate slightly overtime to allow for variation and evolution
10.2 A-B Draw the following structures on page 186 and 187 • Polynucleotide • Sugar phosphate backbone • DNA nucleotide • Pyrimidines • Purines • HW: Exercise 1 in packet