Comparative Study of Free Radical Scavenging and Lipid Peroxidation Inhibition Potentials of Oxythenanthera Abyssinica R
Oxytenanthera abyssinica rhizomes have been used in the treatment of aliments varying from colics, diabetes, polyuria and albuminiura, these ethanomedicinal potencies have been attributed to its rich phytochemical contents and free radical scavenging capacity. This work is aimed at investigating the effect of crude oil pollution on total phenolic, flavonoids and free radical scavenging potentials of methanol extract of Oxytenanthera abyssinica rhizomes. The ability of the plant extracts to scavenge 2,2 diphenyl 1 picylhydrazyl DPPH , hydroxyl radical .OH , superoxide radical O2 , nitric oxide radical NO. and ability to inhibit lipid peroxidation was assayed following standard methods, spectrophotometric method was used to assay for total phenolic and flavonoid content. Non crude oil polluted O. abyssinica extract NCOPOAE and crude oil polluted O. abyssinica extract COPOAE in a concentration dependent manner showed a potent antioxidant activity in DPPH radical scavenging assay EC50= 1.03 u00b10.01u00b5g ml and EC50 = 1.04 u00b11.01u00b5g ml , inhibited hydroxyl radical EC50 = 1.17 u00b11.02u00b5g ml and EC50 = 1.46 u00b11.21u00b5g ml , superoxide anion radical EC50 =1.30 u00b11.21u00b5g ml and EC50 =1.51 u00b11.42u00b5g ml and nitric oxide EC50 = 1.40 u00b11.23u00b5g ml and EC50= 1.53 u00b11.42u00b5g ml also inhibited non enzymatic lipid peroxidation EC50 = 1.58 u00b11.42u00b5g ml and EC50 = 2.47 u00b12.13u00b5g ml and had more of phenolic and flavonoid contents than COPOAE. This result indicates that NCOPOAE contains more antioxidant compounds than COPOAE, which could make it more potent in ethanomedicinal use. Jonathan Chinenye Ifemeje | Kizito Ifeanyi Amaefule | Charles Tochukwu Nwachukwu "Comparative Study of Free Radical Scavenging and Lipid Peroxidation Inhibition Potentials of Oxythenanthera Abyssinica (Rhizomes) Sourced from South Eastern Nigeria" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-6 , October 2019, URL: https://www.ijtsrd.com/papers/ijtsrd29226.pdf Paper URL: https://www.ijtsrd.com/biological-science/biochemistry/29226/comparative-study-of-free-radical-scavenging-and-lipid-peroxidation-inhibition-potentials-of-oxythenanthera-abyssinica-rhizomes-sourced-from-south-eastern-nigeria/jonathan-chinenye-ifemeje
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