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Cryptic Seed Orchard Dysfunctions. The case of Larix decidua x L. kaempferi. Results from Denmark. Danish Seed orchards in production (1947->). Hybrid superiority 25 years from planting, average of 2 sites. Design. Design. 2-5 clones L. decidua. 1 clone L. kaempferi. Design.
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Cryptic Seed Orchard Dysfunctions The case of Larix decidua x L. kaempferi Results from Denmark
Hybrid superiority25 years from planting, average of 2 sites
Design 2-5 clones L. decidua 1 clone L. kaempferi
Design 2-15 clones or Full sib family or Selfed family L. Kaempferi 1 clone L. decidua
Design Hybrids 2-15 clones or Full sib family or Selfed family L. Kaempferi Seed crop Selfed 1 clone L. decidua
Test for hybrids • Allozymes • SSR markers • cpDNA ; mtDNA (Acheré et al. 2004)
Test for hybrids • Allozymes • SSR markers • cpDNA ; mtDNA (Acheré et al. 2004) 601 481
Hybrids? FP 244
Hybrids? FP 205 FP 244
Hybrid superiority25 years from planting, average of 2 sites
Hybrids? FP 205 FP 244
Hybrids? + FP 618 FP 205 FP 244
Hybrids? + FP 618 FP 205 FP 244
Hybrids? + FP 618 FP 203 FP 205 FP 244
Hybrids? + FP 618 FP 203 FP 205 FP 244
Hybrids? + FP 618 FP 203 FP 205 FP 244
Hybrids? + FP 618 FP 203 FP 205 FP 244
Hybrids? + FP 618 FP 211 FP 237 FP 203 FP 201 FP 205 FP 244
Hybrids? Hybrid % in a given Seed Orchards may vary substantially from year to year + FP 203 FP 244 FP 205
Zygotic selectionWhats happends after sowing? • During germination • Growth in the nursery • Culling in the nursery before selling • Growth in the Forests after planting
Zygotic selection - germination time Hybrid fraction according to germination interval FP205 (2004): Hybrid= 63%
Hybrid percent Depends on the sampled germination window … - What is a suitable time window?
Zygotic selection –In the Nursery upto Seedlings Plug+1 Bare roots 11% -> 11% 11% -> 11%
Zygotic selection –In the Nursery upto Seedlings Bare roots 32% -> 45%
Hybrid percent Depends on the applied nursery grading E.g. from 70% – 11% • What is a suitable culling regime? • Should all nurseries test before selling?
Conclusion? • Important to know what’s going on in the CSOs • Test every seedlot • Need for minimum acceptable Hybrid% ? • Need for minimum acceptable culling? • Production technique?
Involved staff Lars Nørgaard Hansen, UoC Erik Dahl Kjær, UoC Viggo Jensen, UoC Lotte Hougs, The Danish Plant Directorate Henrik Gade Knudsen, Thanks to The Danish Forest and Nature Agency for financial support