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Hektoen agar

Hektoen agar . Final Differential and Selective Media. Hektoen Agar. Used to isolate Salmonella and Shigella species from other enterics Based on the ability of bacteria to use three sugars Lactose Sucrose Salcin Ferric ammonium citrate present in media to detect H2S production.

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Hektoen agar

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  1. Hektoen agar Final Differential and Selective Media

  2. Hektoen Agar • Used to isolate Salmonella and Shigella species from other enterics • Based on the ability of bacteria to use three sugars • Lactose • Sucrose • Salcin • Ferric ammonium citrate present in media to detect H2S production

  3. Hektoen Agar (continued) • Bile salts inhibit G+ growth • Bromthymol blue and acid fuchsin are added as color indicators.

  4. Results • Salmomnella, Shigella, and Proteus do not ferment any sugars and produce blue-green colonies • Enteric such as Enterobacter, Escherichia, or Klebsiella produce acid from sugar fermentation and produce yellow to salmon pink colonies • Proteus and Salmonella produce H2S

  5. Lactose fermenter

  6. Lactose non-fermenter

  7. Hektoen comparisonLactose fermenter and non-fermenter

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