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BioChip Arrayer Appendix I1 - BC Array Designer

BioChip Arrayer Appendix I1 - BC Array Designer. NEW OPEN SAVE. ARROW ASPIRATE 4 WELLS ASPIRATE MANY WELLS ZOOM ADD RACK DELETE RACK. ADD LOOP PROPERTIES DELETE SPLIT DECK RULE MAPPING. BioChip Arrayer Using the BC Array Designer. Create a new map.

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BioChip Arrayer Appendix I1 - BC Array Designer

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  1. BioChip ArrayerAppendix I1 -BC Array Designer • NEW • OPEN • SAVE • ARROW • ASPIRATE 4 WELLS • ASPIRATE MANY WELLS • ZOOM • ADD RACK • DELETE RACK • ADD LOOP • PROPERTIES • DELETE • SPLIT DECK • RULE MAPPING

  2. BioChip ArrayerUsing the BC Array Designer • Create a new map

  3. BioChip ArrayerUsing the BC Array Designer • Left side - represents the procedure tree • Right side - represents the BCA deck

  4. BioChip ArrayerUsing the BC Array Designer • Helpful to split the deck so that different zoom percentages can be used

  5. BioChip ArrayerUsing the BC Array Designer • Add source rack and destination racks

  6. BioChip ArrayerUsing the BC Array Designer • Select source rack and destination racks

  7. BioChip ArrayerUsing the BC Array Designer • One source rack and one destination rack have been added

  8. BioChip ArrayerUsing the BC Array Designer • Adjust zoom so that source wells and destination wells are easily seen • Left mouse button magnifies • Right mouse button demagnifies

  9. BioChip ArrayerUsing the BC Array Designer • Zoom has been optimized

  10. BioChip ArrayerUsing the BC Array Designer • Aspirate four wells from source plate and place into wells of destination rack

  11. BioChip ArrayerUsing the BC Array Designer • Click on four wells of interest in source plate

  12. BioChip ArrayerUsing the BC Array Designer • Drag individual source wells to well of choice in destination rack

  13. BioChip ArrayerUsing the BC Array Designer • Aspirate many (in box) wells from source plate and place into wells of destination rack

  14. BioChip ArrayerUsing the BC Array Designer • Choose number of replicates in the column and row direction

  15. BioChip ArrayerUsing the BC Array Designer • Click wells in source plate in box format

  16. BioChip ArrayerUsing the BC Array Designer • Drag box of wells from source rack and place in destination rack in desired location • Distinguish replicates by color

  17. BioChip ArrayerUsing the BC Array Designer • To use the Rule based mapping first select a box of wells

  18. BioChip ArrayerUsing the BC Array Designer • Then click Rule based mapping icon in toolbar.

  19. BioChip ArrayerUsing the BC Array Designer • Select destination rack • Input replicates and number loops

  20. BioChip ArrayerUsing the BC Array Designer • Fills destination wells from left to right starting with the top row

  21. BioChip ArrayerUsing the BC Array Designer • File Test.map has been saved and now can be used in the test to make the array

  22. BioChip ArrayerUsing the BC Array Designer • In BC Table • Create new test • On Rack definition tab use position map • Select Test.map

  23. BioChip ArrayerUsing the BC Array Designer • Notice the Source Rack and Destination rack are filled in • Continue to create the test and run test

  24. BioChip Arrayer™ Flexibility in Array Design and Substrate Choice The array map is defined in the BC Array Designer Software Cy3-labeled dUTP is dispensed onto HydroGel™ 3D substrate

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