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Molecular Methods for Plant Pest/Disease Detection

Molecular Methods for Plant Pest/Disease Detection. Indirect Method. Indirect Method-Biomarker based disease detection. A biomarker is a measurable characteristic in a biological system which changes due to disease, exposure to chemicals or other factors Metabolite profiling

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Molecular Methods for Plant Pest/Disease Detection

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  1. Molecular Methods for Plant Pest/Disease Detection Indirect Method

  2. Indirect Method-Biomarker based disease detection • A biomarker is a measurable characteristic in a biological system which changes due to disease, exposure to chemicals or other factors • Metabolite profiling • measurement of hundreds or potentially thousands of metabolites • GC-MS (Gas Chromatography-Mass Spectrometry) • Compounds are separated by GC and then transferred to the mass spectrometer for further separation and detection

  3. Gas Chromatography-Mass Spectrometry (GC-MS)

  4. Taiz, L & Zeiger, E. 2010. Plant Physiology. 5th Ed. Sinauer Associates, Inc., Publishers. Sunderland-USA.

  5. Indirect Method-Plant properties/stress based detection • Thermal imaging • straightforward choice for visualizing stress-induced changes in leaf transpiration • Imaging spectroscopy • Non destructive • Vegetation has a very characteristic spectral reflectance, or signature caused by chlorophyll absorption • Field based imaging techniques as an alternative to traditional field survey methods for crop disease and pest monitoring

  6. Focon cucumber • Digital infrared thermography (DIT) was employed to detect changes in leaf temperature • Temperature increased as leaves infected by Foc • 10 dpi, necrosis and chlorosis appeared • 11 dpi, leaf dehydration and wild developed http://www.sciencedirect.com/science/article/pii/S0981942812002690

  7. Detection of Fusarium-damaged seeds using spectroscopy technique • Quality control for wheat grain sorting • Hyperspectral imaging (HSI) techniques • Differentiation between 3 type of wheat seeds • Vitreous • Fusarium-damaged • Yellow berry http://www.sciencedirect.com/science/article/pii/S1537511013000196

  8. Things to consider • It is important to remember that molecular diagnostic tests should be used with traditional methods. Molecular tests are not a replacement for the Plant or Insect Diagnostician, but are another tool in the diagnostic process. • Samples should be examined by trained professionals whom can narrow down the list of possible diseases or pests affecting a given host. • Molecular tests should complement traditional methods such as microscopy and culturing. Molecular tests are one component of the diagnostic process.

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