Active compounds extracted from algae for the cosmetic, pharmaceutical and healthfood industries.

1. Active compounds extracted from algae for the cosmetic, pharmaceutical and healthfood industries.

2. The island of OUESSANT, off the west breton coastline

3. The island of OUESSANT, a UNESCO Biosphere Marine Reserve

4. Right in the heart of the largest algae field in Europe

5. Hundreds of algae species

6. YSALINE 100Extracted from the farmed algae Asparagopsis armata

7. YSALINE 100Rich in active halogenous organic compounds Previous research (Codomier, Combault, Mc Donnel, Fenical) => halogenous micromolecules (haloformes, cetones, propanons) ALGUES ET MER innovation : => main activity : macromolecules of MW > 10 000

8. YSALINE 100100% natural extraction process Cold-ground Clarified by tangential microfiltration Cryoconcentrated Freeze-dried

9. YSALINE 100Physico-chemical characteristics Ochre powder with characteristic odour Water soluble pH (5% sol. � 20�C) : 3.0 to 4.5 Dry extract : > 92% Density : 0.6 to 0.8 Additive : none Storage : preserve from light, below 30�C

10. YSALINE 100 Minimal Inhibitory Concentrations Escherichia coli : 0.1 to 0.2% Staphyllococcus aureus : 0.1 to 0.2% Staphyllococcus epidermidis : 0.1 to 0.2% Propionibacterium : 0.1 to 0.2% Pityrosporum ovale : 0.1 to 0.2% Candida albicans : 0.2 to 0.5% Pseudomonas aeruginosa : 0.2 to 0.5% Aspergillus niger : 1.0 to 2.0%

11. YSALINE 100Cosmetic Applications NATURAL PRESERVATIVE - partial or total substitute for existing chemical preservatives (challenge-tests validation necessary) - incorporation levels : 0.2 to 1% ANTI-DANDRUFF - shampoo � regular use � : 0.2 to 0.5% - shampoo � treatment � : 0.5 to 1% ANTI-ACNE and SKIN CLEANSING - skin cleansing : 0.2 to 0.5% - acne treatment : 0.5 to 1%

12. YSALINE 100 Established Toxicological Data Cutaneous irritation . Product tested : 4% solution of YSALINE 100 . Method : EPISKIN + patch-test in vivo / Laboratoire BIOPREDIC . Classification : does not induce histological alteration, non irritating for the skin Ocular irritation . Product tested : 4% solution of YSALINE 100 . Method : PREDISAFE / Laboratoire BIOPREDIC . Classification : slightly irritating, classe I. Mutagenicity . Product tested : 8% solution of YSALINE 100 . Method : AMES Test / Phoenix International Preclinical Services Europe . Classification : no mutagenic effect.

13. DEPOLYMERIZED FUCOIDANSOrigin : Ascophyllum nodosum

14. DEPOLYMERIZED FUCOIDANS The polymer structure

15. DEPOLYMERIZED FUCOIDANS  Process Polyphenols inhibition Extraction of High Molecular Weight Fucoidans Purification and concentration (ultrafiltration) Radical depolymerization (Ac Cu + H202) Second-stage purification (nanofiltration) Drying and grinding

16. DEPOLYMERIZED FUCOIDANS  Physico-chemical characteristics Molecular weight : 8 000 to 12 000 Fucose : 28 to 32 % Uronic acids : 16 to 20 % Sulfates (SO4) : 22 to 26 %

17. DEPOLYMERIZED FUCOIDANS

18. DEPOLYMERIZED FUCOIDANS

19. FUCOIDANS DEPOLYMERIZED

20. DEPOLYMERIZED FUCOIDANS

21. DEPOLYMERIZED FUCOIDANS  Cosmetic applications Moisturizer Anti-ageing Anti-wrinkles Microcirculation enhancer Anti-inflammatory Immune system booster

22. DEPOLYMERIZED FUCOIDANS Toxicological data Acute oral toxicity (OECD guideline n�401) : non toxic /not classified Skin irritation (OECD guideline n�404 and 406) : non irritating /not classified Acute eye irritation (OECD guideline n�405) : slightly irritating /not classified Mutagenicity (OECD guideline n�471): no mutagenic effect

23. POLYPHENOLSOrigin : Ascophyllum nodosum

24. POLYPHENOLSExtraction method / Composition SOLVENT FREE - extraction in aqueous phase at high temperature - alginate free ASCO PP standard - Polyphenols : 5 to 10% - HMW Fucoidans (PM>100 kD) : 40 to 50% - Proteins : 3 to 5% - Lipids : < 1% - Minerals : 30 to 35%

25. POLYPHENOLSApplications Cosmetic and healthfood applications - Anti-oxydant - Anti-radical We are currently elaborating a process for the extraction of free Polyphenols and oligomers.